Synergistic Effect of Hyperglycemia and Suppression on Adult Mouse Islet Beta Cell Replication
The complementary role of hyperglycemia and suppression on islet beta cell regeneration was investigated in a syngeneic mouse model. gene silencing was performed by infecting islets of C57BL/6 with shRNA lentiviral particles. At 54 hours after viral infection, protein content in cultured targetin...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2012/417390 |
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| author | Szu-Tah Chen Shin-Huei Fu Samuel Hsu Yu-Yao Huang Brend Ray-Sea Hsu |
| author_facet | Szu-Tah Chen Shin-Huei Fu Samuel Hsu Yu-Yao Huang Brend Ray-Sea Hsu |
| author_sort | Szu-Tah Chen |
| collection | DOAJ |
| description | The complementary role of hyperglycemia and suppression on islet beta cell regeneration was investigated in a syngeneic mouse model. gene silencing was performed by infecting islets of C57BL/6 with shRNA lentiviral particles. At 54 hours after viral infection, protein content in cultured targeting islets was 22% of that in freshly isolated islets. Six days after transplantation to diabetic mice, targeting islet graft had considerably more cells with Ki67-staining nuclei than nontargeting islets. The mice in the targeting-islet group had a significantly shorter duration of temporary hyperglycaemia than mice in the non-targeting-islet group. The long-term ex vivo beneficial effect of silencing on graft function was also indicated by the significantly higher cumulative cure rate for diabetes in mice receiving 200 targeting islets than that in mice receiving 200 non-targeting islets. Our data suggest that hyperglycemia and persistent suppression have a synergistic effect on islet beta cell replication in adult mice. |
| format | Article |
| id | doaj-art-dbc8aa9a008b4d1baaa1dd5f85dc4548 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-dbc8aa9a008b4d1baaa1dd5f85dc45482025-02-03T06:07:08ZengWileyInternational Journal of Endocrinology1687-83371687-83452012-01-01201210.1155/2012/417390417390Synergistic Effect of Hyperglycemia and Suppression on Adult Mouse Islet Beta Cell ReplicationSzu-Tah Chen0Shin-Huei Fu1Samuel Hsu2Yu-Yao Huang3Brend Ray-Sea Hsu4Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang-Gung Medical Center and Chang-Gung University, Taoyuan 333, TaiwanTaiwan International Graduate Program, Graduate Institute of Life Science, National Defense Medical Center and Academia Sinica, Taipei 115, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Chang-Gung Medical Center and Chang-Gung University, Taoyuan 333, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Chang-Gung Medical Center and Chang-Gung University, Taoyuan 333, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Chang-Gung Medical Center and Chang-Gung University, Taoyuan 333, TaiwanThe complementary role of hyperglycemia and suppression on islet beta cell regeneration was investigated in a syngeneic mouse model. gene silencing was performed by infecting islets of C57BL/6 with shRNA lentiviral particles. At 54 hours after viral infection, protein content in cultured targeting islets was 22% of that in freshly isolated islets. Six days after transplantation to diabetic mice, targeting islet graft had considerably more cells with Ki67-staining nuclei than nontargeting islets. The mice in the targeting-islet group had a significantly shorter duration of temporary hyperglycaemia than mice in the non-targeting-islet group. The long-term ex vivo beneficial effect of silencing on graft function was also indicated by the significantly higher cumulative cure rate for diabetes in mice receiving 200 targeting islets than that in mice receiving 200 non-targeting islets. Our data suggest that hyperglycemia and persistent suppression have a synergistic effect on islet beta cell replication in adult mice.http://dx.doi.org/10.1155/2012/417390 |
| spellingShingle | Szu-Tah Chen Shin-Huei Fu Samuel Hsu Yu-Yao Huang Brend Ray-Sea Hsu Synergistic Effect of Hyperglycemia and Suppression on Adult Mouse Islet Beta Cell Replication International Journal of Endocrinology |
| title | Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication |
| title_full | Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication |
| title_fullStr | Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication |
| title_full_unstemmed | Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication |
| title_short | Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication |
| title_sort | synergistic effect of hyperglycemia and suppression on adult mouse islet beta cell replication |
| url | http://dx.doi.org/10.1155/2012/417390 |
| work_keys_str_mv | AT szutahchen synergisticeffectofhyperglycemiaandsuppressiononadultmouseisletbetacellreplication AT shinhueifu synergisticeffectofhyperglycemiaandsuppressiononadultmouseisletbetacellreplication AT samuelhsu synergisticeffectofhyperglycemiaandsuppressiononadultmouseisletbetacellreplication AT yuyaohuang synergisticeffectofhyperglycemiaandsuppressiononadultmouseisletbetacellreplication AT brendrayseahsu synergisticeffectofhyperglycemiaandsuppressiononadultmouseisletbetacellreplication |