Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice
Exposure to ionizing radiation disrupts metabolic pathways and causes oxidative stress, which can lead to organ damage. In this study, urinary metabolites from mice exposed to high-dose and low-dose whole-body irradiation (WBI HDR, WBI LDR) or partial-body irradiation (PBI BM2.5) were analyzed using...
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| Format: | Article |
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MDPI AG
2024-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/1/24 |
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| author | Yaoxiang Li Shivani Bansal Baldev Singh Meth M. Jayatilake William Klotzbier Marjan Boerma Mi-Heon Lee Jacob Hack Keisuke S. Iwamoto Dörthe Schaue Amrita K. Cheema |
| author_facet | Yaoxiang Li Shivani Bansal Baldev Singh Meth M. Jayatilake William Klotzbier Marjan Boerma Mi-Heon Lee Jacob Hack Keisuke S. Iwamoto Dörthe Schaue Amrita K. Cheema |
| author_sort | Yaoxiang Li |
| collection | DOAJ |
| description | Exposure to ionizing radiation disrupts metabolic pathways and causes oxidative stress, which can lead to organ damage. In this study, urinary metabolites from mice exposed to high-dose and low-dose whole-body irradiation (WBI HDR, WBI LDR) or partial-body irradiation (PBI BM2.5) were analyzed using targeted and untargeted metabolomics approaches. Significant metabolic changes particularly in oxidative stress pathways were observed on Day 2 post-radiation. By Day 30, the WBI HDR group showed persistent metabolic dysregulation, while the WBI LDR and PBI BM2.5 groups were similar to control mice. Machine learning models identified metabolites that were predictive of the type of radiation exposure with high accuracy, highlighting their potential use as biomarkers for radiation damage and oxidative stress. |
| format | Article |
| id | doaj-art-78bd19c9170b47a8a01f706ad68517f7 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-78bd19c9170b47a8a01f706ad68517f72025-01-24T13:19:11ZengMDPI AGAntioxidants2076-39212024-12-011412410.3390/antiox14010024Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in MiceYaoxiang Li0Shivani Bansal1Baldev Singh2Meth M. Jayatilake3William Klotzbier4Marjan Boerma5Mi-Heon Lee6Jacob Hack7Keisuke S. Iwamoto8Dörthe Schaue9Amrita K. Cheema10Department of Oncology, Lombardi Comprehensive Cancer Centre, Georgetown University Medical Center, Washington, DC 20057, USADepartment of Oncology, Lombardi Comprehensive Cancer Centre, Georgetown University Medical Center, Washington, DC 20057, USADepartment of Oncology, Lombardi Comprehensive Cancer Centre, Georgetown University Medical Center, Washington, DC 20057, USADepartment of Oncology, Lombardi Comprehensive Cancer Centre, Georgetown University Medical Center, Washington, DC 20057, USADepartments of Biochemistry, Molecular, and Cellular Biology, Georgetown University Medical Center, Washington, DC 20057, USADivision of Radiation Health, Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Radiation Oncology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90024, USADepartment of Radiation Oncology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90024, USADepartment of Radiation Oncology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90024, USADepartment of Radiation Oncology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90024, USADepartment of Oncology, Lombardi Comprehensive Cancer Centre, Georgetown University Medical Center, Washington, DC 20057, USAExposure to ionizing radiation disrupts metabolic pathways and causes oxidative stress, which can lead to organ damage. In this study, urinary metabolites from mice exposed to high-dose and low-dose whole-body irradiation (WBI HDR, WBI LDR) or partial-body irradiation (PBI BM2.5) were analyzed using targeted and untargeted metabolomics approaches. Significant metabolic changes particularly in oxidative stress pathways were observed on Day 2 post-radiation. By Day 30, the WBI HDR group showed persistent metabolic dysregulation, while the WBI LDR and PBI BM2.5 groups were similar to control mice. Machine learning models identified metabolites that were predictive of the type of radiation exposure with high accuracy, highlighting their potential use as biomarkers for radiation damage and oxidative stress.https://www.mdpi.com/2076-3921/14/1/24radiation exposurewhole-body irradiationpartial-body irradiationmetabolomicsurine metabolomicspathway analysis |
| spellingShingle | Yaoxiang Li Shivani Bansal Baldev Singh Meth M. Jayatilake William Klotzbier Marjan Boerma Mi-Heon Lee Jacob Hack Keisuke S. Iwamoto Dörthe Schaue Amrita K. Cheema Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice Antioxidants radiation exposure whole-body irradiation partial-body irradiation metabolomics urine metabolomics pathway analysis |
| title | Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice |
| title_full | Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice |
| title_fullStr | Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice |
| title_full_unstemmed | Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice |
| title_short | Distinct Urinary Metabolite Signatures Mirror In Vivo Oxidative Stress-Related Radiation Responses in Mice |
| title_sort | distinct urinary metabolite signatures mirror in vivo oxidative stress related radiation responses in mice |
| topic | radiation exposure whole-body irradiation partial-body irradiation metabolomics urine metabolomics pathway analysis |
| url | https://www.mdpi.com/2076-3921/14/1/24 |
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