Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages
In inflammation, the post-transcriptional regulation of transiently expressed genes provides a potential therapeutic target. Tristetraprolin (TTP) is of the factors regulating decay of cytokine mRNAs. The aim of the present study was to identify cytokines whose expression is regulated by TTP. We est...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/40691 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550071279812608 |
|---|---|
| author | Ulla Jalonen Riina Nieminen Katriina Vuolteenaho Hannu Kankaanranta Eeva Moilanen |
| author_facet | Ulla Jalonen Riina Nieminen Katriina Vuolteenaho Hannu Kankaanranta Eeva Moilanen |
| author_sort | Ulla Jalonen |
| collection | DOAJ |
| description | In inflammation, the post-transcriptional regulation of transiently expressed genes provides a potential therapeutic target. Tristetraprolin (TTP) is of the factors regulating decay of cytokine mRNAs. The aim of the present study was to identify cytokines whose expression is regulated by TTP. We established a TTP knock-down cell line by expressing shRNA against TTP (shTTP cell line). A cytokine antibody array was used to measure cytokine production in macrophages exposed to lipopolysaccharide (LPS). Cytokines IL-6, IL-12, TNF-α, and MIP-2 (a homologue to human IL-8) were expressed at higher
levels whereas MIP-3α was produced at lower levels in LPS-treated shTTP cells than in control cells suggesting that the expression of these cytokines is regulated by TTP. The present data provide IL-12, MIP-2, and MIP-3α as novel inflammatory cytokine targets for TTP-mediated mRNA decay and stress the role of TTP in the regulation of the inflammatory process. |
| format | Article |
| id | doaj-art-65fb0657e4ad4255afa4a6e7b727b4d9 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-65fb0657e4ad4255afa4a6e7b727b4d92025-02-03T06:07:48ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/4069140691Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated MacrophagesUlla Jalonen0Riina Nieminen1Katriina Vuolteenaho2Hannu Kankaanranta3Eeva Moilanen4The Immunopharmacology Research Group, Medical School, University of Tampere, and Tampere University Hospital, Research Unit, Tampere 33014, FinlandThe Immunopharmacology Research Group, Medical School, University of Tampere, and Tampere University Hospital, Research Unit, Tampere 33014, FinlandThe Immunopharmacology Research Group, Medical School, University of Tampere, and Tampere University Hospital, Research Unit, Tampere 33014, FinlandThe Immunopharmacology Research Group, Medical School, University of Tampere, and Tampere University Hospital, Research Unit, Tampere 33014, FinlandThe Immunopharmacology Research Group, Medical School, University of Tampere, and Tampere University Hospital, Research Unit, Tampere 33014, FinlandIn inflammation, the post-transcriptional regulation of transiently expressed genes provides a potential therapeutic target. Tristetraprolin (TTP) is of the factors regulating decay of cytokine mRNAs. The aim of the present study was to identify cytokines whose expression is regulated by TTP. We established a TTP knock-down cell line by expressing shRNA against TTP (shTTP cell line). A cytokine antibody array was used to measure cytokine production in macrophages exposed to lipopolysaccharide (LPS). Cytokines IL-6, IL-12, TNF-α, and MIP-2 (a homologue to human IL-8) were expressed at higher levels whereas MIP-3α was produced at lower levels in LPS-treated shTTP cells than in control cells suggesting that the expression of these cytokines is regulated by TTP. The present data provide IL-12, MIP-2, and MIP-3α as novel inflammatory cytokine targets for TTP-mediated mRNA decay and stress the role of TTP in the regulation of the inflammatory process.http://dx.doi.org/10.1155/MI/2006/40691 |
| spellingShingle | Ulla Jalonen Riina Nieminen Katriina Vuolteenaho Hannu Kankaanranta Eeva Moilanen Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages Mediators of Inflammation |
| title | Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages |
| title_full | Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages |
| title_fullStr | Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages |
| title_full_unstemmed | Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages |
| title_short | Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages |
| title_sort | down regulation of tristetraprolin expression results in enhanced il 12 and mip 2 production and reduced mip 3α synthesis in activated macrophages |
| url | http://dx.doi.org/10.1155/MI/2006/40691 |
| work_keys_str_mv | AT ullajalonen downregulationoftristetraprolinexpressionresultsinenhancedil12andmip2productionandreducedmip3asynthesisinactivatedmacrophages AT riinanieminen downregulationoftristetraprolinexpressionresultsinenhancedil12andmip2productionandreducedmip3asynthesisinactivatedmacrophages AT katriinavuolteenaho downregulationoftristetraprolinexpressionresultsinenhancedil12andmip2productionandreducedmip3asynthesisinactivatedmacrophages AT hannukankaanranta downregulationoftristetraprolinexpressionresultsinenhancedil12andmip2productionandreducedmip3asynthesisinactivatedmacrophages AT eevamoilanen downregulationoftristetraprolinexpressionresultsinenhancedil12andmip2productionandreducedmip3asynthesisinactivatedmacrophages |