Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs
<b>Background/Objectives:</b> The endosomal escape of lipid nanoparticles (LNPs) is crucial for efficient mRNA-based therapeutics. Here, we present a cationic polymeric micelle (cPM) as a safe and potent co-delivery system with enhanced endosomal escape capabilities. <b>Methods:<...
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2024-12-01
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| author | Siyuan Deng Han Shao Hongtao Shang Lingjin Pang Xiaomeng Chen Jingyi Cao Yi Wang Zhao Zhao |
| author_facet | Siyuan Deng Han Shao Hongtao Shang Lingjin Pang Xiaomeng Chen Jingyi Cao Yi Wang Zhao Zhao |
| author_sort | Siyuan Deng |
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| description | <b>Background/Objectives:</b> The endosomal escape of lipid nanoparticles (LNPs) is crucial for efficient mRNA-based therapeutics. Here, we present a cationic polymeric micelle (cPM) as a safe and potent co-delivery system with enhanced endosomal escape capabilities. <b>Methods:</b> We synthesized a cationic and ampholytic di-block copolymer, poly (poly (ethylene glycol)<sub>4-5</sub> methacrylate<sub>a</sub>-<i>co</i>-hexyl methacrylate<sub>b</sub>)<sub>X</sub>-<i>b</i>-poly(butyl methacrylate<sub>c</sub>-<i>co</i>-dimethylaminoethyl methacrylate<sub>d</sub>-<i>co</i>-propyl acrylate<sub>e</sub>)<sub>Y</sub> (p(PEG<sub>4-5</sub>MA<sub>a</sub>-<i>co</i>-HMA<sub>b</sub>)<sub>X</sub>-<i>b</i>-p(BMA<sub>c</sub>-<i>co</i>-DMAEMA<sub>d</sub>-<i>co</i>-PAA<sub>e</sub>)<sub>Y</sub>), via reversible addition–fragmentation chain transfer polymerization. The cPMs were then formulated using the synthesized polymer by the dispersion–diffusion method and characterized by dynamic light scattering (DLS) and cryo-transmission electron microscopy (CryoTEM). The membrane-destabilization activity of the cPMs was evaluated by a hemolysis assay. We performed an in vivo functional assay of firefly luciferase (Fluc) mRNA using two of the most commonly studied LNPs, SM102 LNP and Dlin-MC3-DMA LNPs. <b>Results:</b> With a particle size of 61.31 ± 0.68 nm and a zeta potential of 37.76 ± 2.18 mV, the cPMs exhibited a 2–3 times higher firefly luciferase signal at the injection site compared to the control groups without cPMs following intramuscular injection in mice, indicating the high potential of cPMs to enhance the endosomal escape efficiency of mRNA-LNPs. <b>Conclusions:</b> The developed cPM, with enhanced endosomal escape capabilities, presents a promising strategy to improve the expression efficiency of delivered mRNAs. This approach offers a novel alternative strategy with no modifications to the inherent properties of mRNA-LNPs, preventing any unforeseeable changes in formulation characteristics. Consequently, this polymer-based nanomaterial holds immense potential for clinical applications in mRNA-based vaccines. |
| format | Article |
| id | doaj-art-52822fbe35534b6db361f1e7a4cb79b6 |
| institution | Kabale University |
| issn | 2076-393X |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-52822fbe35534b6db361f1e7a4cb79b62025-01-24T13:51:42ZengMDPI AGVaccines2076-393X2024-12-011312510.3390/vaccines13010025Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPsSiyuan Deng0Han Shao1Hongtao Shang2Lingjin Pang3Xiaomeng Chen4Jingyi Cao5Yi Wang6Zhao Zhao7Shenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, ChinaShenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, ChinaShenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, ChinaShenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, ChinaShenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, ChinaShenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, ChinaShenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, ChinaShenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, China<b>Background/Objectives:</b> The endosomal escape of lipid nanoparticles (LNPs) is crucial for efficient mRNA-based therapeutics. Here, we present a cationic polymeric micelle (cPM) as a safe and potent co-delivery system with enhanced endosomal escape capabilities. <b>Methods:</b> We synthesized a cationic and ampholytic di-block copolymer, poly (poly (ethylene glycol)<sub>4-5</sub> methacrylate<sub>a</sub>-<i>co</i>-hexyl methacrylate<sub>b</sub>)<sub>X</sub>-<i>b</i>-poly(butyl methacrylate<sub>c</sub>-<i>co</i>-dimethylaminoethyl methacrylate<sub>d</sub>-<i>co</i>-propyl acrylate<sub>e</sub>)<sub>Y</sub> (p(PEG<sub>4-5</sub>MA<sub>a</sub>-<i>co</i>-HMA<sub>b</sub>)<sub>X</sub>-<i>b</i>-p(BMA<sub>c</sub>-<i>co</i>-DMAEMA<sub>d</sub>-<i>co</i>-PAA<sub>e</sub>)<sub>Y</sub>), via reversible addition–fragmentation chain transfer polymerization. The cPMs were then formulated using the synthesized polymer by the dispersion–diffusion method and characterized by dynamic light scattering (DLS) and cryo-transmission electron microscopy (CryoTEM). The membrane-destabilization activity of the cPMs was evaluated by a hemolysis assay. We performed an in vivo functional assay of firefly luciferase (Fluc) mRNA using two of the most commonly studied LNPs, SM102 LNP and Dlin-MC3-DMA LNPs. <b>Results:</b> With a particle size of 61.31 ± 0.68 nm and a zeta potential of 37.76 ± 2.18 mV, the cPMs exhibited a 2–3 times higher firefly luciferase signal at the injection site compared to the control groups without cPMs following intramuscular injection in mice, indicating the high potential of cPMs to enhance the endosomal escape efficiency of mRNA-LNPs. <b>Conclusions:</b> The developed cPM, with enhanced endosomal escape capabilities, presents a promising strategy to improve the expression efficiency of delivered mRNAs. This approach offers a novel alternative strategy with no modifications to the inherent properties of mRNA-LNPs, preventing any unforeseeable changes in formulation characteristics. Consequently, this polymer-based nanomaterial holds immense potential for clinical applications in mRNA-based vaccines.https://www.mdpi.com/2076-393X/13/1/25mRNA-LNPcationic polymeric micelleendosomal escapetransfection efficiency |
| spellingShingle | Siyuan Deng Han Shao Hongtao Shang Lingjin Pang Xiaomeng Chen Jingyi Cao Yi Wang Zhao Zhao Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs Vaccines mRNA-LNP cationic polymeric micelle endosomal escape transfection efficiency |
| title | Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs |
| title_full | Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs |
| title_fullStr | Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs |
| title_full_unstemmed | Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs |
| title_short | Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs |
| title_sort | development of a cationic polymeric micellar structure with endosomal escape capability enables enhanced intramuscular transfection of mrna lnps |
| topic | mRNA-LNP cationic polymeric micelle endosomal escape transfection efficiency |
| url | https://www.mdpi.com/2076-393X/13/1/25 |
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