Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by <i>Escherichia coli</i>
The low solubility of CO<sub>2</sub> in water leads to massive CO<sub>2</sub> emission and extremely low CO<sub>2</sub> utilization in succinic acid (SA) biosynthesis. To enhance microbial CO<sub>2</sub> utilization, micro-nano bubbles (MNBs) were indu...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/1/31 |
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| Summary: | The low solubility of CO<sub>2</sub> in water leads to massive CO<sub>2</sub> emission and extremely low CO<sub>2</sub> utilization in succinic acid (SA) biosynthesis. To enhance microbial CO<sub>2</sub> utilization, micro-nano bubbles (MNBs) were induced in SA biosynthesis by <i>E. coli</i> Suc260 in this study. The results showed that MNB aeration decreased CO<sub>2</sub> emissions and increased CO<sub>2</sub> solubility in the medium significantly. The CO<sub>2</sub> utilization of MNB aeration was 129.69% higher than that of bubble aeration in atmospheric fermentation. However, MNBs showed a significant inhibitory effect on bacterial growth in the pressurized environment, although a two-stage aerobic–anaerobic fermentation strategy weakened the inhibition. The biofilm-enhanced strain <i>E. coli</i> Suc260-CsgA showed a strong tolerance to MNBs. In pressurized fermentation with MNB aeration, the actual CO<sub>2</sub> utilization of <i>E. coli</i> Suc260-CsgA was 30.63% at 0.18 MPa, which was a 6.49-times improvement. The CO<sub>2</sub> requirement for SA synthesis decreased by 83.4%, and the fugitive emission of CO<sub>2</sub> was successfully controlled. The activities of key enzymes within the SA synthesis pathway were also maintained or enhanced in the fermentation process with MNB aeration. These results indicated that the biofilm-enhanced strain and CO<sub>2</sub>-MNBs could improve carbon fixation efficiency in microbial carbon sequestration. |
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| ISSN: | 2311-5637 |