CircOMA1调控多巴胺受体促进泌乳素瘤耐药性
目的探究环状RNA hsa_circ_0002316(circOMA1)是否参与泌乳素瘤耐药调控及其作用机制是什么。方法通过RT-qPCR检测临床泌乳素瘤标本中circOMA1表达情况(5例敏感泌乳素瘤标本和12例耐药泌乳素瘤标本)。通过慢病毒载体构建稳定表达外源性circOMA1 MMQ细胞系模型进行体外实验,分为MMQ NC组及MMQ OMA1组。通过构建裸鼠皮下肿瘤模型进行体内实验,分为MMQ NC+卡麦角林(CAB)组、MMQ NC+溴隐亭(BRC)组、MMQ OMA1+CAB组、MMQ OMA1+BRC组。通过CCK8、Western blot、ELISA等表型实验检测circOMA...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | zho |
Published: |
Editorial Office of Journal of Sun Yat-sen University
2022-05-01
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Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
Subjects: | |
Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/doi/10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2022.0306/ |
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