Establishment of an indirect ELISA method for detecting bovine coronavirus antibodies based on N protein

Establishment of an indirect ELISA method for detecting bovine coronavirus antibodies based on N protein

Bovine Coronavirus (BCoV) is a significant pathogen responsible for neonatal calf diarrhea, winter dysentery in adult cattle, and bovine respiratory diseases. Infection with the virus can result in hemorrhagic diarrhea, decreased milk production, and potentially fatal outcomes in cattle, leading to...

Full description

Saved in:

Bibliographic Details
Main Authors:	Qiang Liu, Xiaoxia Niu, Lingling Jiang, Gang Zhang, Pu Wang, Sinong Zhang, Weifeng Gao, Huichen Guo, Yujiong Wang, Yong Li
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-02-01
Series:	Frontiers in Veterinary Science
Subjects:	bovine coronavirus N protein eukaryotic expression indirect ELISA antibody detection
Online Access:	https://www.frontiersin.org/articles/10.3389/fvets.2025.1530870/full
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

Bayesian Estimation of the True Bovine Brucellosis Prevalence in Vaccinated and Non-Vaccinated Ecuadorian Cattle Populations, and the Sensitivity and Specificity of a Competitive and Indirect ELISA Using a New Synthetic Antigen
by: Ana Dolores Garrido Haro, et al.
Published: (2025-01-01)

Evaluation of IgG binding capability to SARS-CoV-2 variants in early COVID-19 convalescent sera using an indirect ELISA
by: Behzad Hussain, et al.
Published: (2025-02-01)

Cross-sectional seroprevalence study of bovine herpesvirus 1, bovine respiratory syncytial virus, and parainfluenza virus 3 in cattle from Villavicencio, Colombia
by: Catalina Ballesteros González, et al.
Published: (2025-01-01)

INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA
by: A. S. Yakovleva, et al.
Published: (2018-10-01)

TEST-SYSTEM BASED ON INDIRECT “SANDWICH” ELISA TO DETECT THE LUMPY SKIN DISEASE VIRUS ANTIGEN
by: O. A. Ryabikina, et al.
Published: (2018-10-01)

Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs
by: Francisco Jesus Castañeda Montes, et al.
Published: (2024-11-01)

Identification of fine antigenic epitopes of Tamdy virus glycoprotein Gn fragment and establishment of ELISA detection method
by: Yujiao Fu, et al.
Published: (2025-01-01)

The investigation of marek disease, lenfoid leucosis and reticuloendotheliosis by elisa and histopathologic methods in chickens in konya province
by: Uçkun Sait Uçan, et al.

Enzyme-linked immunosorbent assay for post-slaughter diagnosis of bovine leukosis
by: A. R. Mustafayev, et al.
Published: (2024-06-01)

Evaluation the Efficacy of ELISA IgG, IgM and IgA Tests for Diagnosis of Helicobacter pylori
by: Hogir Mohammed Shukri Saadi, et al.
Published: (2018-09-01)

EFFECTIVENESS OF SEROLOGICAL TESTS FOR DIAGNOSTICS OF BOVINE EPHEMERAL FEVER
by: Ye. A. Konstantinova, et al.
Published: (2018-04-01)

Genome-Wide Genomic Analysis and Evolutionary Insights into Bovine Coronavirus Strains in Southwest China
by: Qingqing Li, et al.
Published: (2024-12-01)

AN ERROR ANALYSIS OF THE TRANSFORMATION FROM DIRECT TO INDIRECT SPEECH
by: Zahratul Idami
Published: (2018-01-01)

AGLUTINACIÓN EN LÁTEX, ELISA Y HEMOAGLUTINACIÓN: ALTERNATIVAS PARA EL DIAGNÓSTICO DE LA PARVOVIROSIS CANINA EN HECES
by: S Ariza, et al.
Published: (2005-01-01)

Indirection argumentative et mythologie grecque dans l’essai d’Albert Camus
by: Bahia Zemni, et al.
Published: (2025-02-01)

Serological and virological investigation of bovine viral diarrhea virus infection in cattle with abortion problem
by: Oya Bulut, et al.

Diagnosis of bovine viral diarrhea in Bubalus bubalis dairy farms
by: Oriana Gavidia, et al.
Published: (2023-11-01)

Global boundedness in a Keller-Segel system with nonlinear indirect signal consumption mechanism
by: Zihan Zheng, et al.
Published: (2024-08-01)

Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa
by: N. N. Lugovskaya, et al.
Published: (2018-04-01)

Seropositivity for Bovine Viral Diarrhea and Enzootic Bovine Leukemia viruses in Blanco Orejinegro cattle in Colombia and infection associated-factors
by: A. Saldarriaga-Saldarriaga, et al.
Published: (2021-01-01)

Seroprevalence and Associated Risk Factors of Infectious Bovine Rhinotracheitis (IBR) and Animal Owners’ Knowledge, Attitude and Practice (KAP) Towards the Disease in Selected Districts of East Wollega Zone, Oromia Regional State, Ethiopia
by: Abdisa Mitiku Danu, et al.
Published: (2024-11-01)

Effect of site of insemination on bovine fertility
by: Mehmet B. Ataman, et al.

Assessment of different enrichment methods revealed the optimal approach to identify bovine circRnas
by: Yixin Wang, et al.
Published: (2024-12-01)

Proteomics and metabolomics analyses of mechanism underlying bovine sperm cryoinjury
by: Renzheng Zhang, et al.
Published: (2025-01-01)

Comparison of Resting Energy Expenditure Using Indirect Calorimetry and Predictive Equations in Trauma Patients: A Pilot Study
by: Dae Sung Ma, et al.
Published: (2021-03-01)

Analysis of global dynamics in an attraction-repulsion model with nonlinear indirect signal and logistic source
by: Chang-Jian Wang, et al.
Published: (2024-10-01)

Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera
by: Ye. S. Kostrova, et al.
Published: (2018-04-01)

Methodological considerations for estimating indirect costs in children and adolescents with chronic conditions: a scoping review
by: Anne Kitschen, et al.
Published: (2025-01-01)

Investigating Diagnostic Accuracy of Strip Kit (Rapid Test) for Detecting Aflatoxin M1 in Milk Based on the Concentration Obtained by ELISA and HPLC
by: Hanieh Amiri, et al.
Published: (2025-02-01)

Detection and Validation of A2 Milk Suitable for Consumers Having Milk Intolerance by ELISA Method
by: Mediha Esra Yayla
Published: (2023-12-01)

Mirror Target YOLO: An Improved YOLOv8 Method With Indirect Vision for Heritage Buildings Fire Detection
by: Jian Liang, et al.
Published: (2025-01-01)

Antibiotic resistances of enterococci isolated from bovine subclinical mastitis
by: Yahya Kuyucuoğlu

Prevalence and associated risk factors of bovine Schistosoma and Fasciola infections among cattle in South Achefer District, North West Ethiopia
by: Solomon Tesfaye, et al.
Published: (2025-05-01)

Bovine leukemia virus occurrence in Dagestan
by: N. R. Budulov, et al.
Published: (2023-06-01)

Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates
by: Ligiane de Oliveira Leme, et al.
Published: (2025-02-01)

Unexplained Air Disasters: Compensation for Indirect Victims
by: Jean Michel Haziza
Published: (2024-12-01)

Distinct ecological processes shape micro-eukaryotic generalists and specialists in a canal-disturbed wetland, Lake Weishan, China
by: Xinghao Li, et al.
Published: (2025-01-01)

Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages
by: Lucas José Barone, et al.
Published: (2024-12-01)

Ultrasound‐assisted minimally invasive technique for tissue collection from bovine mammary glands
by: Alysia L. Vang, et al.
Published: (2024-11-01)

Indirect Multicultural Experiences: A Multidimensional Journey to Inspire Self-Change
by: Jun Zhang, et al.
Published: (2025-01-01)