Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticus
The aims of this research were to isolate and identify pathogenic proteolytic bacteria from external body part of GIFT tilapia and to study the role of the selected bacteria. Twenty bacterial isolates that were incubated at room temperature (25-27ºC) revealed proteolytic activity in nutrient agar c...
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| Format: | Article |
| Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2009-07-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/4101 |
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| author | D. Wahjuningrum L. Mayasari N.R. Mubarik |
| author_facet | D. Wahjuningrum L. Mayasari N.R. Mubarik |
| author_sort | D. Wahjuningrum |
| collection | DOAJ |
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The aims of this research were to isolate and identify pathogenic proteolytic bacteria from external body part of GIFT tilapia and to study the role of the selected bacteria. Twenty bacterial isolates that were incubated at room temperature (25-27ºC) revealed proteolytic activity in nutrient agar containing 0.5% skimmed milk. Staphylococcus sp strain T.c2 and Necromonas sp.strain T.s2 isolates from infected tilapia were selected based on the proteolytic index. Postulat Koch test showed that Necromonas sp. strain T.s2. was pathogenic bacteria for tilapia and has a high value of proteolytic index.
Keywords : Tilapia, Oreochromis niloticus, proteolytic bacteria, pathogen.
ABSTRAK
Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri proteolitik patogen dari bagian eksternal ikan nila strain GIFT serta untuk mempelajari peranan dari bakteri proteolitik terpilih. Duapuluh isolat bakteri yang diinkubasi pada suhu ruang (25-27ºC) menunjukkan aktivitas proteolitik dalam media agar yang mengandung 0,5% susu skim. Berdasarkan indeks proteolitik tertinggi, diidentifikasi dua isolat yaitu Staphylococcus sp. strain T.c2 dan Necromonas sp. .strain T.s2 dari ikan nila yang terinfeksi. Uji Postulat Koch menunjukkan bahwa isolat Necromonas sp.strain T.s2 bersifat patogen bagi ikan nila dan memiliki indeks proteolitik yang tinggi.
Kata kunci : Ikan nila, Oreochromis niloticus, bakteri proteolitik, patogen.
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| format | Article |
| id | doaj-art-fedde95ffc1146d89314a2830d0b9bdc |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2009-07-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-fedde95ffc1146d89314a2830d0b9bdc2025-01-24T18:01:50ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002009-07-018210.19027/jai.8.169-1743699Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticusD. Wahjuningrum0L. Mayasari1N.R. Mubarik2Bogor Agricultural University, Department of AquacultureDepartemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Darmaga BogorDepartemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Jalan Raya Pajajaran, Bogor The aims of this research were to isolate and identify pathogenic proteolytic bacteria from external body part of GIFT tilapia and to study the role of the selected bacteria. Twenty bacterial isolates that were incubated at room temperature (25-27ºC) revealed proteolytic activity in nutrient agar containing 0.5% skimmed milk. Staphylococcus sp strain T.c2 and Necromonas sp.strain T.s2 isolates from infected tilapia were selected based on the proteolytic index. Postulat Koch test showed that Necromonas sp. strain T.s2. was pathogenic bacteria for tilapia and has a high value of proteolytic index. Keywords : Tilapia, Oreochromis niloticus, proteolytic bacteria, pathogen. ABSTRAK Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri proteolitik patogen dari bagian eksternal ikan nila strain GIFT serta untuk mempelajari peranan dari bakteri proteolitik terpilih. Duapuluh isolat bakteri yang diinkubasi pada suhu ruang (25-27ºC) menunjukkan aktivitas proteolitik dalam media agar yang mengandung 0,5% susu skim. Berdasarkan indeks proteolitik tertinggi, diidentifikasi dua isolat yaitu Staphylococcus sp. strain T.c2 dan Necromonas sp. .strain T.s2 dari ikan nila yang terinfeksi. Uji Postulat Koch menunjukkan bahwa isolat Necromonas sp.strain T.s2 bersifat patogen bagi ikan nila dan memiliki indeks proteolitik yang tinggi. Kata kunci : Ikan nila, Oreochromis niloticus, bakteri proteolitik, patogen. https://journal.ipb.ac.id/index.php/jai/article/view/4101 |
| spellingShingle | D. Wahjuningrum L. Mayasari N.R. Mubarik Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticus Jurnal Akuakultur Indonesia |
| title | Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticus |
| title_full | Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticus |
| title_fullStr | Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticus |
| title_full_unstemmed | Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticus |
| title_short | Isolation and Identification of Pathogenic Proteolytic Bacteria from External Body Part of GIFT Tilapia Oreochromis niloticus |
| title_sort | isolation and identification of pathogenic proteolytic bacteria from external body part of gift tilapia oreochromis niloticus |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/4101 |
| work_keys_str_mv | AT dwahjuningrum isolationandidentificationofpathogenicproteolyticbacteriafromexternalbodypartofgifttilapiaoreochromisniloticus AT lmayasari isolationandidentificationofpathogenicproteolyticbacteriafromexternalbodypartofgifttilapiaoreochromisniloticus AT nrmubarik isolationandidentificationofpathogenicproteolyticbacteriafromexternalbodypartofgifttilapiaoreochromisniloticus |