Development and Characterization of the NanoOrange® Protein Quantitation Assay: A Fluorescence-Based Assay of Proteins in Solution
We developed a sensitive fluorescence assay for the quantitation of proteins in solution using the NanoOrange® reagent, a merocyanine dye that produces a large increase in fluorescence quantum yield upon interaction with detergent-coated proteins. The NanoOrange assay allowed for the detection of 10...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03344pt03 |
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