iRFP is a real time marker for transformation based assays in high content screening.
Anchorage independent growth is one of the hallmarks of oncogenic transformation. Here we show that infrared fluorescent protein (iRFP) based assays allow accurate and unbiased determination of colony formation and anchorage independent growth over time. This protocol is particularly compatible with...
Saved in:
| Main Authors: | Viola Paulus-Hock, Eric C Cheung, Patricia Roxburgh, Karen H Vousden, Andreas K Hock |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0098399 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Screening for Hepatocellular Carcinoma
by: Hock-Foong Lui
Published: (2011-01-01) -
Apoptosis assessment in high-content and high-throughput screening assays
by: Céline Rens, et al.
Published: (2021-06-01) -
A real-time assay for neutrophil chemotaxis
by: Patricia M. Cano, et al.
Published: (2016-05-01) -
An image-based high-content screening assay for compounds targeting intracellular Leishmania donovani amastigotes in human macrophages.
by: Jair L Siqueira-Neto, et al.
Published: (2012-01-01) -
Elif MAHIR METINSOY, Ottoman Women during World War I: everyday experiences, politics, and conflict
by: Stefan Hock
Published: (2022-09-01)