Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp
This study focuses on gene expression patterns and functions in human umbilical cord (UC) and dental pulp (DP) containing mesenchymal stem cells (MSCs). DP tissues were collected from 25 permanent premolars. UC tissue samples were obtained from three newborns. Comparative gene profiles were obtained...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/3453890 |
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| _version_ | 1832548165599887360 |
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| author | Chung-Min Kang Hyunok Kim Je Seon Song Byung-Jai Choi Seong-Oh Kim Han-Sung Jung Seok-Jun Moon Hyung-Jun Choi |
| author_facet | Chung-Min Kang Hyunok Kim Je Seon Song Byung-Jai Choi Seong-Oh Kim Han-Sung Jung Seok-Jun Moon Hyung-Jun Choi |
| author_sort | Chung-Min Kang |
| collection | DOAJ |
| description | This study focuses on gene expression patterns and functions in human umbilical cord (UC) and dental pulp (DP) containing mesenchymal stem cells (MSCs). DP tissues were collected from 25 permanent premolars. UC tissue samples were obtained from three newborns. Comparative gene profiles were obtained using cDNA microarray analysis and the expression of tooth development-associated and MSC-related genes was assessed by the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Genes related to cell proliferation, angiogenesis, and immune responses were expressed at higher levels in UC, whereas genes related to growth factor and receptor activity and signal transduction were more highly expressed in DP. Although UC and DP tissues exhibited similar expression of surface markers for MSCs, UC showed higher expression of CD29, CD34, CD44, CD73, CD105, CD146, and CD166. qRT-PCR analysis showed that CD146, CD166, and MYC were expressed 18.3, 8.24, and 1.63 times more highly in UC, whereas the expression of CD34 was 2.15 times higher in DP. Immunohistochemical staining revealed significant differences in the expression of genes (DSPP, DMP1, and CALB1) related to odontogenesis and angiogenesis in DP. DP and UC tissue showed similar gene expression, with the usual MSC markers, while they clearly diverged in their differentiation capacity. |
| format | Article |
| id | doaj-art-fbc8dc474ea042ecb4b3b46b00bd4f34 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-fbc8dc474ea042ecb4b3b46b00bd4f342025-02-03T06:42:14ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/34538903453890Genetic Comparison of Stemness of Human Umbilical Cord and Dental PulpChung-Min Kang0Hyunok Kim1Je Seon Song2Byung-Jai Choi3Seong-Oh Kim4Han-Sung Jung5Seok-Jun Moon6Hyung-Jun Choi7Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of KoreaDepartment of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of KoreaDepartment of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of KoreaDepartment of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of KoreaDepartment of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of KoreaDepartment of Oral Biology, Division of Histology, College of Dentistry, Yonsei University, Seoul, Republic of KoreaDepartment of Oral Biology, Division of Pharmacology, College of Dentistry, Yonsei University, Seoul, Republic of KoreaDepartment of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of KoreaThis study focuses on gene expression patterns and functions in human umbilical cord (UC) and dental pulp (DP) containing mesenchymal stem cells (MSCs). DP tissues were collected from 25 permanent premolars. UC tissue samples were obtained from three newborns. Comparative gene profiles were obtained using cDNA microarray analysis and the expression of tooth development-associated and MSC-related genes was assessed by the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Genes related to cell proliferation, angiogenesis, and immune responses were expressed at higher levels in UC, whereas genes related to growth factor and receptor activity and signal transduction were more highly expressed in DP. Although UC and DP tissues exhibited similar expression of surface markers for MSCs, UC showed higher expression of CD29, CD34, CD44, CD73, CD105, CD146, and CD166. qRT-PCR analysis showed that CD146, CD166, and MYC were expressed 18.3, 8.24, and 1.63 times more highly in UC, whereas the expression of CD34 was 2.15 times higher in DP. Immunohistochemical staining revealed significant differences in the expression of genes (DSPP, DMP1, and CALB1) related to odontogenesis and angiogenesis in DP. DP and UC tissue showed similar gene expression, with the usual MSC markers, while they clearly diverged in their differentiation capacity.http://dx.doi.org/10.1155/2016/3453890 |
| spellingShingle | Chung-Min Kang Hyunok Kim Je Seon Song Byung-Jai Choi Seong-Oh Kim Han-Sung Jung Seok-Jun Moon Hyung-Jun Choi Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp Stem Cells International |
| title | Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp |
| title_full | Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp |
| title_fullStr | Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp |
| title_full_unstemmed | Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp |
| title_short | Genetic Comparison of Stemness of Human Umbilical Cord and Dental Pulp |
| title_sort | genetic comparison of stemness of human umbilical cord and dental pulp |
| url | http://dx.doi.org/10.1155/2016/3453890 |
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