Fast and precise multi-site mutagenesis on linear DNA fragments

The effectiveness and scalability of site-directed mutagenesis are constrained by the limited number of mutations and the intricate cloning process required for isolation of the target sequence. Here, we present a method for precise introduction of multiple non-contiguous mutations. The mutant stran...

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Bibliographic Details
Main Authors: Jie Zhang, Youhui Yang, Zhaoguan Wang, Qian Liu, Peixian Li, Shaohua Kang, Hao Qi
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Biotechnology & Biotechnological Equipment
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Online Access:https://www.tandfonline.com/doi/10.1080/13102818.2024.2385423
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