<i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology
Saline–alkali environments restrict soybean production in China. Wild soybean genes can be used to improve the alkaline tolerance of cultivated soybean in molecular breeding. The expansin protein family promotes cell wall expansion. In this study, the relative expression levels of expansin family ge...
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2024-12-01
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| author | Mengyu Liu Jixiang Tang Shengjie Ma Yujing Liu Xiaoyu Wang Xinlei Du Xiaohuan Sun Yucheng Zeng Yulong Zeng Xiaodong Ding Yingpeng Han Junfeng Zhang Lei Cao |
| author_facet | Mengyu Liu Jixiang Tang Shengjie Ma Yujing Liu Xiaoyu Wang Xinlei Du Xiaohuan Sun Yucheng Zeng Yulong Zeng Xiaodong Ding Yingpeng Han Junfeng Zhang Lei Cao |
| author_sort | Mengyu Liu |
| collection | DOAJ |
| description | Saline–alkali environments restrict soybean production in China. Wild soybean genes can be used to improve the alkaline tolerance of cultivated soybean in molecular breeding. The expansin protein family promotes cell wall expansion. In this study, the relative expression levels of expansin family genes in wild soybean treated with 50 mM NaHCO<sub>3</sub> were measured at 0, 3, 6, and 12 h, and the relative expression of <i>GsEXPA8</i> was found to be higher at 12 h. Wild soybean was treated with abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA), and jasmonic acid (JA), and <i>GsEXPA8</i> was found to respond to ABA and IAA signals. Sequence analysis shows that <i>GsEXPA8</i> has DPBB_EXPA and expansin domains. Subcellular localization analysis shows that <i>GsEXPA8</i> was localized in the cytoplasm in protoplasts and the cell membrane or wall in tobacco, indicating that it has nuclear membrane localization signals. <i>GsEXPA8</i> overexpression reduced the malondialdehyde content in transgenic plants treated with NaHCO<sub>3</sub> and increased peroxidase activity before treatment. After the transformation of soybean roots from hair roots, <i>GsEXPA8</i> was found to be expressed in the outer root cells and promote the development of thicker, shorter roots, thereby improving the plant’s alkaline tolerance. Stable <i>GsEXPA8</i> transformation improved saline alkaline tolerance via the regulation of the alkali stress-related genes <i>GmKIN1</i>, <i>GmRD22</i>, <i>GmDnaJA6</i>, <i>GmNFYC1</i>, and <i>GmMYB14</i>. These findings provide support for further research on alkali-tolerance regulation pathways and molecular breeding for alkali tolerance. |
| format | Article |
| id | doaj-art-fb8c59b52312485f82e5aad3f660d47f |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-fb8c59b52312485f82e5aad3f660d47f2025-01-24T13:16:22ZengMDPI AGAgronomy2073-43952024-12-011511610.3390/agronomy15010016<i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root MorphologyMengyu Liu0Jixiang Tang1Shengjie Ma2Yujing Liu3Xiaoyu Wang4Xinlei Du5Xiaohuan Sun6Yucheng Zeng7Yulong Zeng8Xiaodong Ding9Yingpeng Han10Junfeng Zhang11Lei Cao12College of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaHangzhou Fusheng Agricultural Technology Development Co., Ltd., No.1, Yinhu Street, Fuyang District, Hangzhou 311400, ChinaHangzhou Fusheng Agricultural Technology Development Co., Ltd., No.1, Yinhu Street, Fuyang District, Hangzhou 311400, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaSchool of Geography and Tourism, Harbin University, No. 109, Zhongxing Avenue, Nangang District, Harbin 150076, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin 150030, ChinaSaline–alkali environments restrict soybean production in China. Wild soybean genes can be used to improve the alkaline tolerance of cultivated soybean in molecular breeding. The expansin protein family promotes cell wall expansion. In this study, the relative expression levels of expansin family genes in wild soybean treated with 50 mM NaHCO<sub>3</sub> were measured at 0, 3, 6, and 12 h, and the relative expression of <i>GsEXPA8</i> was found to be higher at 12 h. Wild soybean was treated with abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA), and jasmonic acid (JA), and <i>GsEXPA8</i> was found to respond to ABA and IAA signals. Sequence analysis shows that <i>GsEXPA8</i> has DPBB_EXPA and expansin domains. Subcellular localization analysis shows that <i>GsEXPA8</i> was localized in the cytoplasm in protoplasts and the cell membrane or wall in tobacco, indicating that it has nuclear membrane localization signals. <i>GsEXPA8</i> overexpression reduced the malondialdehyde content in transgenic plants treated with NaHCO<sub>3</sub> and increased peroxidase activity before treatment. After the transformation of soybean roots from hair roots, <i>GsEXPA8</i> was found to be expressed in the outer root cells and promote the development of thicker, shorter roots, thereby improving the plant’s alkaline tolerance. Stable <i>GsEXPA8</i> transformation improved saline alkaline tolerance via the regulation of the alkali stress-related genes <i>GmKIN1</i>, <i>GmRD22</i>, <i>GmDnaJA6</i>, <i>GmNFYC1</i>, and <i>GmMYB14</i>. These findings provide support for further research on alkali-tolerance regulation pathways and molecular breeding for alkali tolerance.https://www.mdpi.com/2073-4395/15/1/16<i>Glycine soja</i><i>Glycine max</i><i>GsEXPA8</i>alkaline stressroot morphogenesis |
| spellingShingle | Mengyu Liu Jixiang Tang Shengjie Ma Yujing Liu Xiaoyu Wang Xinlei Du Xiaohuan Sun Yucheng Zeng Yulong Zeng Xiaodong Ding Yingpeng Han Junfeng Zhang Lei Cao <i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology Agronomy <i>Glycine soja</i> <i>Glycine max</i> <i>GsEXPA8</i> alkaline stress root morphogenesis |
| title | <i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology |
| title_full | <i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology |
| title_fullStr | <i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology |
| title_full_unstemmed | <i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology |
| title_short | <i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology |
| title_sort | i gsexpa8 i enhances soybean tolerance of nahco sub 3 sub stress by regulating root morphology |
| topic | <i>Glycine soja</i> <i>Glycine max</i> <i>GsEXPA8</i> alkaline stress root morphogenesis |
| url | https://www.mdpi.com/2073-4395/15/1/16 |
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