<i>GsEXPA8</i> Enhances Soybean Tolerance of NaHCO<sub>3</sub> Stress by Regulating Root Morphology
Saline–alkali environments restrict soybean production in China. Wild soybean genes can be used to improve the alkaline tolerance of cultivated soybean in molecular breeding. The expansin protein family promotes cell wall expansion. In this study, the relative expression levels of expansin family ge...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/1/16 |
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| Summary: | Saline–alkali environments restrict soybean production in China. Wild soybean genes can be used to improve the alkaline tolerance of cultivated soybean in molecular breeding. The expansin protein family promotes cell wall expansion. In this study, the relative expression levels of expansin family genes in wild soybean treated with 50 mM NaHCO<sub>3</sub> were measured at 0, 3, 6, and 12 h, and the relative expression of <i>GsEXPA8</i> was found to be higher at 12 h. Wild soybean was treated with abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA), and jasmonic acid (JA), and <i>GsEXPA8</i> was found to respond to ABA and IAA signals. Sequence analysis shows that <i>GsEXPA8</i> has DPBB_EXPA and expansin domains. Subcellular localization analysis shows that <i>GsEXPA8</i> was localized in the cytoplasm in protoplasts and the cell membrane or wall in tobacco, indicating that it has nuclear membrane localization signals. <i>GsEXPA8</i> overexpression reduced the malondialdehyde content in transgenic plants treated with NaHCO<sub>3</sub> and increased peroxidase activity before treatment. After the transformation of soybean roots from hair roots, <i>GsEXPA8</i> was found to be expressed in the outer root cells and promote the development of thicker, shorter roots, thereby improving the plant’s alkaline tolerance. Stable <i>GsEXPA8</i> transformation improved saline alkaline tolerance via the regulation of the alkali stress-related genes <i>GmKIN1</i>, <i>GmRD22</i>, <i>GmDnaJA6</i>, <i>GmNFYC1</i>, and <i>GmMYB14</i>. These findings provide support for further research on alkali-tolerance regulation pathways and molecular breeding for alkali tolerance. |
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| ISSN: | 2073-4395 |