Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular Carcinoma
Pregenomic RNA (pgRNA) is generated from covalently closed circular DNA (cccDNA) and plays important roles in viral genome amplification and replication. Hepatic pgRNA and cccDNA expression levels indicate viral persistence and replication activity. This study was aimed to measure hepatic pgRNA and...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2013/849290 |
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| author | Fugui Bai Yoshihiko Yano Takumi Fukumoto Atsushi Takebe Motofumi Tanaka Kaori Kuramitsu Nungki Anggorowati Hanggoro Tri Rinonce Dewiyani Indah Widasari Masaya Saito Hirotaka Hirano Takanobu Hayakumo Yasushi Seo Takeshi Azuma Yonson Ku Yoshitake Hayashi |
| author_facet | Fugui Bai Yoshihiko Yano Takumi Fukumoto Atsushi Takebe Motofumi Tanaka Kaori Kuramitsu Nungki Anggorowati Hanggoro Tri Rinonce Dewiyani Indah Widasari Masaya Saito Hirotaka Hirano Takanobu Hayakumo Yasushi Seo Takeshi Azuma Yonson Ku Yoshitake Hayashi |
| author_sort | Fugui Bai |
| collection | DOAJ |
| description | Pregenomic RNA (pgRNA) is generated from covalently closed circular DNA (cccDNA) and plays important roles in viral genome amplification and replication. Hepatic pgRNA and cccDNA expression levels indicate viral persistence and replication activity. This study was aimed to measure hepatic pgRNA and cccDNA expression levels in various states of hepatitis B virus (HBV) infection. Thirty-eight hepatocellular carcinoma (HCC) patients, including 14 positive for hepatitis B surface antigen (HBsAg) and 24 negative for HBsAg but positive for anti-hepatitis B core (anti-HBc) antibody, were enrolled in this study. In HBsAg-negative but anti-HBc-positive group, HBV-DNA was detected in 20 of 24 (83%) noncancerous liver tissues for at least two genomic regions based on polymerase chain reaction (PCR) analysis. pgRNA and cccDNA expression levels in occult HBV-infected patients were significantly lower than those in HBsAg-positive patients (P<0.001). pgRNA and cccDNA in cancerous tissues were also detected without significant difference from those in noncancerous tissues. In conclusion, cccDNA and pgRNA are detected and represented HBV replication not only in noncancerous but also in cancerous liver tissues. In addition, the replication is shown in not only patients with HBsAg-positive but also occult HBV-infected patients, suggesting the contribution to HCC development. |
| format | Article |
| id | doaj-art-faf48e08392c41d3a36b494945d79adb |
| institution | Kabale University |
| issn | 2090-3448 2090-3456 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hepatology |
| spelling | doaj-art-faf48e08392c41d3a36b494945d79adb2025-02-03T01:23:33ZengWileyInternational Journal of Hepatology2090-34482090-34562013-01-01201310.1155/2013/849290849290Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular CarcinomaFugui Bai0Yoshihiko Yano1Takumi Fukumoto2Atsushi Takebe3Motofumi Tanaka4Kaori Kuramitsu5Nungki Anggorowati6Hanggoro Tri Rinonce7Dewiyani Indah Widasari8Masaya Saito9Hirotaka Hirano10Takanobu Hayakumo11Yasushi Seo12Takeshi Azuma13Yonson Ku14Yoshitake Hayashi15Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanCenter for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanCenter for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanCenter for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanCenter for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Gastroenterology, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Gastroenterology, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Gastroenterology, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Gastroenterology, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Gastroenterology, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanDepartment of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanCenter for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe 650-0017, JapanPregenomic RNA (pgRNA) is generated from covalently closed circular DNA (cccDNA) and plays important roles in viral genome amplification and replication. Hepatic pgRNA and cccDNA expression levels indicate viral persistence and replication activity. This study was aimed to measure hepatic pgRNA and cccDNA expression levels in various states of hepatitis B virus (HBV) infection. Thirty-eight hepatocellular carcinoma (HCC) patients, including 14 positive for hepatitis B surface antigen (HBsAg) and 24 negative for HBsAg but positive for anti-hepatitis B core (anti-HBc) antibody, were enrolled in this study. In HBsAg-negative but anti-HBc-positive group, HBV-DNA was detected in 20 of 24 (83%) noncancerous liver tissues for at least two genomic regions based on polymerase chain reaction (PCR) analysis. pgRNA and cccDNA expression levels in occult HBV-infected patients were significantly lower than those in HBsAg-positive patients (P<0.001). pgRNA and cccDNA in cancerous tissues were also detected without significant difference from those in noncancerous tissues. In conclusion, cccDNA and pgRNA are detected and represented HBV replication not only in noncancerous but also in cancerous liver tissues. In addition, the replication is shown in not only patients with HBsAg-positive but also occult HBV-infected patients, suggesting the contribution to HCC development.http://dx.doi.org/10.1155/2013/849290 |
| spellingShingle | Fugui Bai Yoshihiko Yano Takumi Fukumoto Atsushi Takebe Motofumi Tanaka Kaori Kuramitsu Nungki Anggorowati Hanggoro Tri Rinonce Dewiyani Indah Widasari Masaya Saito Hirotaka Hirano Takanobu Hayakumo Yasushi Seo Takeshi Azuma Yonson Ku Yoshitake Hayashi Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular Carcinoma International Journal of Hepatology |
| title | Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular Carcinoma |
| title_full | Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular Carcinoma |
| title_fullStr | Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular Carcinoma |
| title_full_unstemmed | Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular Carcinoma |
| title_short | Quantification of Pregenomic RNA and Covalently Closed Circular DNA in Hepatitis B Virus-Related Hepatocellular Carcinoma |
| title_sort | quantification of pregenomic rna and covalently closed circular dna in hepatitis b virus related hepatocellular carcinoma |
| url | http://dx.doi.org/10.1155/2013/849290 |
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