Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility
Spherical biocarriers were prepared with lignin-carbohydrate complexes isolated from ginkgo (Ginkgo biloba L.) xylem. The specific surface and average pore size of the biocarriers were 17.15 m2 g−1 and 21.59 nm, respectively. The carriers were stable in solution at pH 4.0~9.5. Fourier transform infr...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2017/4915185 |
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| author | Houkuan Zhao Jinling Li Peng Wang Shaoqiong Zeng Yimin Xie |
| author_facet | Houkuan Zhao Jinling Li Peng Wang Shaoqiong Zeng Yimin Xie |
| author_sort | Houkuan Zhao |
| collection | DOAJ |
| description | Spherical biocarriers were prepared with lignin-carbohydrate complexes isolated from ginkgo (Ginkgo biloba L.) xylem. The specific surface and average pore size of the biocarriers were 17.15 m2 g−1 and 21.59 nm, respectively. The carriers were stable in solution at pH 4.0~9.5. Fourier transform infrared (FT-IR) spectrum indicated that the spherical carrier was composed of lignin and polysaccharides and had a typical lignin-carbohydrate complex (LCC) structure. The contents of galactose, lignin, and total sugar were 3.30%, 23.9%, and 64.62%, respectively, making the spherical biocarriers have good physical strength and compatible with hepatocytes. It was observed using a scanning electron microscopy (SEM) that liver cells adhered to the spherical biocarriers during culture. Cell counting indicated that the proliferation of liver cells in the experimental group was significantly higher than that of the control group. The albumin secretion (ALB) value and glucose consumption of the human hepatocytes were increased by 51.7% and 38.6%, respectively, by the fourth day when cultivated on the biocarriers. The results indicate that ginkgo LCC is very biocompatible and shows promise for the use as a biomaterial in the culture of human hepatocytes. |
| format | Article |
| id | doaj-art-fad73600dafc4da18d59d2b1e8db1c63 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | International Journal of Polymer Science |
| spelling | doaj-art-fad73600dafc4da18d59d2b1e8db1c632025-02-03T06:00:07ZengWileyInternational Journal of Polymer Science1687-94221687-94302017-01-01201710.1155/2017/49151854915185Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and BiocompatibilityHoukuan Zhao0Jinling Li1Peng Wang2Shaoqiong Zeng3Yimin Xie4Research Institute of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, ChinaResearch Institute of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, ChinaResearch Institute of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, ChinaResearch Institute of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, ChinaResearch Institute of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, ChinaSpherical biocarriers were prepared with lignin-carbohydrate complexes isolated from ginkgo (Ginkgo biloba L.) xylem. The specific surface and average pore size of the biocarriers were 17.15 m2 g−1 and 21.59 nm, respectively. The carriers were stable in solution at pH 4.0~9.5. Fourier transform infrared (FT-IR) spectrum indicated that the spherical carrier was composed of lignin and polysaccharides and had a typical lignin-carbohydrate complex (LCC) structure. The contents of galactose, lignin, and total sugar were 3.30%, 23.9%, and 64.62%, respectively, making the spherical biocarriers have good physical strength and compatible with hepatocytes. It was observed using a scanning electron microscopy (SEM) that liver cells adhered to the spherical biocarriers during culture. Cell counting indicated that the proliferation of liver cells in the experimental group was significantly higher than that of the control group. The albumin secretion (ALB) value and glucose consumption of the human hepatocytes were increased by 51.7% and 38.6%, respectively, by the fourth day when cultivated on the biocarriers. The results indicate that ginkgo LCC is very biocompatible and shows promise for the use as a biomaterial in the culture of human hepatocytes.http://dx.doi.org/10.1155/2017/4915185 |
| spellingShingle | Houkuan Zhao Jinling Li Peng Wang Shaoqiong Zeng Yimin Xie Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility International Journal of Polymer Science |
| title | Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility |
| title_full | Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility |
| title_fullStr | Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility |
| title_full_unstemmed | Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility |
| title_short | Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility |
| title_sort | lignin carbohydrate complexes based spherical biocarriers preparation characterization and biocompatibility |
| url | http://dx.doi.org/10.1155/2017/4915185 |
| work_keys_str_mv | AT houkuanzhao lignincarbohydratecomplexesbasedsphericalbiocarrierspreparationcharacterizationandbiocompatibility AT jinlingli lignincarbohydratecomplexesbasedsphericalbiocarrierspreparationcharacterizationandbiocompatibility AT pengwang lignincarbohydratecomplexesbasedsphericalbiocarrierspreparationcharacterizationandbiocompatibility AT shaoqiongzeng lignincarbohydratecomplexesbasedsphericalbiocarrierspreparationcharacterizationandbiocompatibility AT yiminxie lignincarbohydratecomplexesbasedsphericalbiocarrierspreparationcharacterizationandbiocompatibility |