Protocol for the purification of the plastid-encoded RNA polymerase from transplastomic tobacco plants

Protocol for the purification of the plastid-encoded RNA polymerase from transplastomic tobacco plants

Summary: The plastid-encoded RNA polymerase (PEP) plays an essential role in the transcription of the chloroplast genome. Here, we present a strategy to purify the transcriptionally active protein complex from transplastomic tobacco (Nicotiana tabacum) lines in which one of the PEP core subunits is...

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Bibliographic Details
Main Authors: Xiao-Xian Wu, Fan Li, Chuxia Zhu, Shu-Yi Sun, Wen-Hui Mu, Stephanie Ruf, Ralph Bock, Yu Zhang, Fei Zhou
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Molecular Biology
Plant sciences
Protein Biochemistry
Structural Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006932
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