Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
We showed that internalization of Streptococcus uberis into bovine mammary epithelial cells occurred through receptor- (RME) and caveolae-mediated endocytosis (CME). We reported also that treatment of S. uberis with host proteins including lactoferrin (LF) enhanced its internalization into host cell...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2010/319192 |
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| author | Raul A. Almeida John R. Dunlap Stephen P. Oliver |
| author_facet | Raul A. Almeida John R. Dunlap Stephen P. Oliver |
| author_sort | Raul A. Almeida |
| collection | DOAJ |
| description | We showed that internalization of Streptococcus uberis into bovine mammary epithelial cells occurred through receptor- (RME) and caveolae-mediated endocytosis (CME). We reported also that treatment of S. uberis with host proteins including lactoferrin (LF) enhanced its internalization into host cells. Since the underlying mechanism(s) involved in such enhancement was unknown we investigated if preincubation of S. uberis with host proteins drives internalization of this pathogen into host cells through CME. Thus, experiments involving coculture of collagen-, fibronectin-, and LF-pretreated S. uberis with bovine mammary epithelial cells treated with RME and CME inhibitors were conducted. Results showed that internalization of host proteins-pretreated S. uberis into mammary epithelial cells treated with RME inhibitors was higher than that of untreated controls. These results suggest that pretreatment with selected host proteins commits S. uberis to CME, thus avoiding intracellular bactericidal mechanisms and allowing its persistence into bovine mammary epithelial cells. |
| format | Article |
| id | doaj-art-fa30e22ac2974722a6b8e7d53b97e803 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-fa30e22ac2974722a6b8e7d53b97e8032025-02-03T01:03:08ZengWileyVeterinary Medicine International2042-00482010-01-01201010.4061/2010/319192319192Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial CellsRaul A. Almeida0John R. Dunlap1Stephen P. Oliver2Department of Animal Science, The Food Safety Center of Excellence, The University of Tennessee, Knoxville, TN 37996, USADepartment of Botany, The University of Tennessee, Knoxville, TN 37996, USADepartment of Animal Science, The Food Safety Center of Excellence, The University of Tennessee, Knoxville, TN 37996, USAWe showed that internalization of Streptococcus uberis into bovine mammary epithelial cells occurred through receptor- (RME) and caveolae-mediated endocytosis (CME). We reported also that treatment of S. uberis with host proteins including lactoferrin (LF) enhanced its internalization into host cells. Since the underlying mechanism(s) involved in such enhancement was unknown we investigated if preincubation of S. uberis with host proteins drives internalization of this pathogen into host cells through CME. Thus, experiments involving coculture of collagen-, fibronectin-, and LF-pretreated S. uberis with bovine mammary epithelial cells treated with RME and CME inhibitors were conducted. Results showed that internalization of host proteins-pretreated S. uberis into mammary epithelial cells treated with RME inhibitors was higher than that of untreated controls. These results suggest that pretreatment with selected host proteins commits S. uberis to CME, thus avoiding intracellular bactericidal mechanisms and allowing its persistence into bovine mammary epithelial cells.http://dx.doi.org/10.4061/2010/319192 |
| spellingShingle | Raul A. Almeida John R. Dunlap Stephen P. Oliver Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells Veterinary Medicine International |
| title | Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells |
| title_full | Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells |
| title_fullStr | Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells |
| title_full_unstemmed | Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells |
| title_short | Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells |
| title_sort | binding of host factors influences internalization and intracellular trafficking of streptococcus uberis in bovine mammary epithelial cells |
| url | http://dx.doi.org/10.4061/2010/319192 |
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