Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice
The activation of Toll-Like Receptor-3 (TLR3) and Toll-Like Receptor-4 (TLR4) signalling pathways is a regular pathway for immune system activation during infection. This study aimed to investigate the effects of vitamin D (VD) and Tinospora cordifolia ethanol extract (TC) on TLR3 and TLR4 receptor...
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/05/bioconf_icgrc2025_03008.pdf |
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| author | Jamil Ahmad Shobrun Widyarti Sri Setiawan Meddy Rifa’i Muhaimin |
| author_facet | Jamil Ahmad Shobrun Widyarti Sri Setiawan Meddy Rifa’i Muhaimin |
| author_sort | Jamil Ahmad Shobrun |
| collection | DOAJ |
| description | The activation of Toll-Like Receptor-3 (TLR3) and Toll-Like Receptor-4 (TLR4) signalling pathways is a regular pathway for immune system activation during infection. This study aimed to investigate the effects of vitamin D (VD) and Tinospora cordifolia ethanol extract (TC) on TLR3 and TLR4 receptor protein expression, proinflammatory cytokine (IL1 and IL-6) production, and antimicrobial peptide cathelicidin (CAP) production in CD11b+ cells of mice infected with Escherichia coli. The treatments consisted of administration of VD (0.325 µg/kg bw), TC (100 mg/kg bw), and a combination of both in the same dose for 28 days, followed by induction of E. coli infection on day 29. The flow cytometry method was analyzed of TLR3, TLR4, IL-1, IL-6, and CAP expression in CD11b+ cells of experimental animals. The following measurement results were compared with healthy controls and infected animals with the significance of differences between treatments analyzed by One-way ANOVA with p < 0.05. The results showed that administering VD, TC, and a combination of both reduced the expression of TLR3, TLR4, and IL-1 compared to treating infected animals. The combination treatment of VD + TC increased CAP production more than all other treatments. This significant finding suggests that the combination of VD + TC has the potential to control inflammation without disrupting the body’s defence mechanisms against infection, providing valuable insights for the field of immunology. |
| format | Article |
| id | doaj-art-fa06616606d240748528127bd86677b9 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-fa06616606d240748528127bd86677b92025-02-05T10:43:07ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011540300810.1051/bioconf/202515403008bioconf_icgrc2025_03008Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected miceJamil Ahmad Shobrun0Widyarti Sri1Setiawan Meddy2Rifa’i Muhaimin3Student of the Doctoral Program, Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya UniversityDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya UniversityDepartment of Medicine Education, Faculty of Medicine, University of MuhammadiyahDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya UniversityThe activation of Toll-Like Receptor-3 (TLR3) and Toll-Like Receptor-4 (TLR4) signalling pathways is a regular pathway for immune system activation during infection. This study aimed to investigate the effects of vitamin D (VD) and Tinospora cordifolia ethanol extract (TC) on TLR3 and TLR4 receptor protein expression, proinflammatory cytokine (IL1 and IL-6) production, and antimicrobial peptide cathelicidin (CAP) production in CD11b+ cells of mice infected with Escherichia coli. The treatments consisted of administration of VD (0.325 µg/kg bw), TC (100 mg/kg bw), and a combination of both in the same dose for 28 days, followed by induction of E. coli infection on day 29. The flow cytometry method was analyzed of TLR3, TLR4, IL-1, IL-6, and CAP expression in CD11b+ cells of experimental animals. The following measurement results were compared with healthy controls and infected animals with the significance of differences between treatments analyzed by One-way ANOVA with p < 0.05. The results showed that administering VD, TC, and a combination of both reduced the expression of TLR3, TLR4, and IL-1 compared to treating infected animals. The combination treatment of VD + TC increased CAP production more than all other treatments. This significant finding suggests that the combination of VD + TC has the potential to control inflammation without disrupting the body’s defence mechanisms against infection, providing valuable insights for the field of immunology.https://www.bio-conferences.org/articles/bioconf/pdf/2025/05/bioconf_icgrc2025_03008.pdf |
| spellingShingle | Jamil Ahmad Shobrun Widyarti Sri Setiawan Meddy Rifa’i Muhaimin Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice BIO Web of Conferences |
| title | Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice |
| title_full | Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice |
| title_fullStr | Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice |
| title_full_unstemmed | Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice |
| title_short | Vitamin D and Tinospora cordifolia modulate TLR3 and TLR4 pathways, reduce inflammation, and maintain antimicrobial peptide levels in infected mice |
| title_sort | vitamin d and tinospora cordifolia modulate tlr3 and tlr4 pathways reduce inflammation and maintain antimicrobial peptide levels in infected mice |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/05/bioconf_icgrc2025_03008.pdf |
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