Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late period
This study evaluated the effects of Parachlorella sp. KSN1 (PA) supplementation on the gut microbiota and intestinal immunity of broilers of different ages. A total of 180 Ross 308 broiler chicks were weighed and divided into early (1 to 10 days post hatch) and late (11 to 28 days post hatch) period...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011507 |
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| author | Woonhak Ji Tae-Yong Kim Chae Won Lee Z-Hun Kim Ji Young Jung Byeong Cheol Ban Changsu Kong Myunghoo Kim |
| author_facet | Woonhak Ji Tae-Yong Kim Chae Won Lee Z-Hun Kim Ji Young Jung Byeong Cheol Ban Changsu Kong Myunghoo Kim |
| author_sort | Woonhak Ji |
| collection | DOAJ |
| description | This study evaluated the effects of Parachlorella sp. KSN1 (PA) supplementation on the gut microbiota and intestinal immunity of broilers of different ages. A total of 180 Ross 308 broiler chicks were weighed and divided into early (1 to 10 days post hatch) and late (11 to 28 days post hatch) periods, with six replicates of 10 chicks per cage assigned to two dietary groups. The experimental diets included a corn-soybean meal-based control diet and a treatment diet supplemented with 0.5% PA, replacing corn or corn starch, and fed ad libitum for the assigned experimental period. On days 10 and 28, two broilers from each of the six replicate cages, with 7 broilers per cage in each group, were selected and euthanized, and cecal feces and intestinal tissue samples were collected. PA supplementation did not significantly affect broilers growth performance during both the early and the late periods. However, PA supplementation altered the cecal microbiome, with Clostridiaceae and Clostridium exhibiting prominent and consistent changes. In terms of intestinal immunity, PA supplementation significantly increased the number of CD3+ and CD4+ T cells when administered only during the early period. Cecal IgA levels were significantly increased by PA supplementation during both the early and late periods. A significant positive correlation was observed between IgA, Clostridiaceae and Clostridium during the early and late periods. Gene expression analysis identified 40 upregulated genes, including polymeric immunoglobulin receptor (pIgR), and 142 downregulated genes, including marginal zone B and B1 cell specific protein and immunoglobulin lambda-like polypeptide 1 that were associated with the IgA response in PA-treated broilers during the early period. This study demonstrated that PA supplementation promotes gut microbial colonization and intestinal immunity development during the early age of broilers. These findings suggest that the early growth period of broilers is the optimal time for dietary immunomodulation to promote gut health in broilers. |
| format | Article |
| id | doaj-art-f7473de1a60148a08cfcb1d526be2be0 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-f7473de1a60148a08cfcb1d526be2be02025-01-22T05:40:32ZengElsevierPoultry Science0032-57912025-01-011041104572Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late periodWoonhak Ji0Tae-Yong Kim1Chae Won Lee2Z-Hun Kim3Ji Young Jung4Byeong Cheol Ban5Changsu Kong6Myunghoo Kim7Department of Animal Science, College of Natural Resources & Live Science, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Animal Science, College of Natural Resources & Live Science, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Republic of KoreaHu Evergreen Pharm Corp., 164 Yeorumul-ro, Bupyeong-gu, Incheon 21445, Republic of KoreaBiological Resources Research Department, Nakdonggang National Institute of Biological Resources (NNIBR), Sangju 37242, Republic of KoreaDepartment of Animal Science, College of Natural Resources & Live Science, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Republic of Korea; Department of Animal Science, Kyungpook National University, Sangju 37224, Republic of Korea; Research Institute for Innovative Animal Science, Kyungpook National University, Sangju 37224, Republic of Korea; Corresponding author at: Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Republic of Korea.Department of Animal Science, College of Natural Resources & Live Science, Pusan National University, Miryang 50463, Republic of Korea; Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea; Future Earth Research Institute, PNU JYS Science Academy, Pusan National University, Busan 46241, South Korea; Corresponding author at: Department of Animal Science, College of Natural Resources & Live Science, Pusan National University, Miryang 50463, Republic of Korea.This study evaluated the effects of Parachlorella sp. KSN1 (PA) supplementation on the gut microbiota and intestinal immunity of broilers of different ages. A total of 180 Ross 308 broiler chicks were weighed and divided into early (1 to 10 days post hatch) and late (11 to 28 days post hatch) periods, with six replicates of 10 chicks per cage assigned to two dietary groups. The experimental diets included a corn-soybean meal-based control diet and a treatment diet supplemented with 0.5% PA, replacing corn or corn starch, and fed ad libitum for the assigned experimental period. On days 10 and 28, two broilers from each of the six replicate cages, with 7 broilers per cage in each group, were selected and euthanized, and cecal feces and intestinal tissue samples were collected. PA supplementation did not significantly affect broilers growth performance during both the early and the late periods. However, PA supplementation altered the cecal microbiome, with Clostridiaceae and Clostridium exhibiting prominent and consistent changes. In terms of intestinal immunity, PA supplementation significantly increased the number of CD3+ and CD4+ T cells when administered only during the early period. Cecal IgA levels were significantly increased by PA supplementation during both the early and late periods. A significant positive correlation was observed between IgA, Clostridiaceae and Clostridium during the early and late periods. Gene expression analysis identified 40 upregulated genes, including polymeric immunoglobulin receptor (pIgR), and 142 downregulated genes, including marginal zone B and B1 cell specific protein and immunoglobulin lambda-like polypeptide 1 that were associated with the IgA response in PA-treated broilers during the early period. This study demonstrated that PA supplementation promotes gut microbial colonization and intestinal immunity development during the early age of broilers. These findings suggest that the early growth period of broilers is the optimal time for dietary immunomodulation to promote gut health in broilers.http://www.sciencedirect.com/science/article/pii/S0032579124011507BroilerMicroalgaeGut healthGut microbiomeGut immunity |
| spellingShingle | Woonhak Ji Tae-Yong Kim Chae Won Lee Z-Hun Kim Ji Young Jung Byeong Cheol Ban Changsu Kong Myunghoo Kim Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late period Poultry Science Broiler Microalgae Gut health Gut microbiome Gut immunity |
| title | Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late period |
| title_full | Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late period |
| title_fullStr | Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late period |
| title_full_unstemmed | Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late period |
| title_short | Supplementation of Parachlorella sp. in feed promote the gut microbiome colonization and fecal IgA response of broiler in both early and late period |
| title_sort | supplementation of parachlorella sp in feed promote the gut microbiome colonization and fecal iga response of broiler in both early and late period |
| topic | Broiler Microalgae Gut health Gut microbiome Gut immunity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011507 |
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