Automatic Generation of Validated Specific Epitope Sets
Accurate measurement of B and T cell responses is a valuable tool to study autoimmunity, allergies, immunity to pathogens, and host-pathogen interactions and assist in the design and evaluation of T cell vaccines and immunotherapies. In this context, it is desirable to elucidate a method to select v...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/763461 |
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| author | Sebastian Carrasco Pro John Sidney Sinu Paul Cecilia Lindestam Arlehamn Daniela Weiskopf Bjoern Peters Alessandro Sette |
| author_facet | Sebastian Carrasco Pro John Sidney Sinu Paul Cecilia Lindestam Arlehamn Daniela Weiskopf Bjoern Peters Alessandro Sette |
| author_sort | Sebastian Carrasco Pro |
| collection | DOAJ |
| description | Accurate measurement of B and T cell responses is a valuable tool to study autoimmunity, allergies, immunity to pathogens, and host-pathogen interactions and assist in the design and evaluation of T cell vaccines and immunotherapies. In this context, it is desirable to elucidate a method to select validated reference sets of epitopes to allow detection of T and B cells. However, the ever-growing information contained in the Immune Epitope Database (IEDB) and the differences in quality and subjects studied between epitope assays make this task complicated. In this study, we develop a novel method to automatically select reference epitope sets according to a categorization system employed by the IEDB. From the sets generated, three epitope sets (EBV, mycobacteria and dengue) were experimentally validated by detection of T cell reactivity ex vivo from human donors. Furthermore, a web application that will potentially be implemented in the IEDB was created to allow users the capacity to generate customized epitope sets. |
| format | Article |
| id | doaj-art-f64e530d503d4d40bbf6209b232b1c7c |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-f64e530d503d4d40bbf6209b232b1c7c2025-02-03T06:11:41ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/763461763461Automatic Generation of Validated Specific Epitope SetsSebastian Carrasco Pro0John Sidney1Sinu Paul2Cecilia Lindestam Arlehamn3Daniela Weiskopf4Bjoern Peters5Alessandro Sette6Laboratorio de Bioinformática y Biología Molecular, Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, PeruDivision of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USADivision of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USADivision of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USADivision of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USADivision of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USADivision of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USAAccurate measurement of B and T cell responses is a valuable tool to study autoimmunity, allergies, immunity to pathogens, and host-pathogen interactions and assist in the design and evaluation of T cell vaccines and immunotherapies. In this context, it is desirable to elucidate a method to select validated reference sets of epitopes to allow detection of T and B cells. However, the ever-growing information contained in the Immune Epitope Database (IEDB) and the differences in quality and subjects studied between epitope assays make this task complicated. In this study, we develop a novel method to automatically select reference epitope sets according to a categorization system employed by the IEDB. From the sets generated, three epitope sets (EBV, mycobacteria and dengue) were experimentally validated by detection of T cell reactivity ex vivo from human donors. Furthermore, a web application that will potentially be implemented in the IEDB was created to allow users the capacity to generate customized epitope sets.http://dx.doi.org/10.1155/2015/763461 |
| spellingShingle | Sebastian Carrasco Pro John Sidney Sinu Paul Cecilia Lindestam Arlehamn Daniela Weiskopf Bjoern Peters Alessandro Sette Automatic Generation of Validated Specific Epitope Sets Journal of Immunology Research |
| title | Automatic Generation of Validated Specific Epitope Sets |
| title_full | Automatic Generation of Validated Specific Epitope Sets |
| title_fullStr | Automatic Generation of Validated Specific Epitope Sets |
| title_full_unstemmed | Automatic Generation of Validated Specific Epitope Sets |
| title_short | Automatic Generation of Validated Specific Epitope Sets |
| title_sort | automatic generation of validated specific epitope sets |
| url | http://dx.doi.org/10.1155/2015/763461 |
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