NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i>

NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i>

<i>Vairimorpha (Nosema) ceranae</i> is a pathogen that affects <i>Apis mellifera</i> and <i>Apis ceranae</i> Fabricius, capable of spreading within and between honeybee colonies. The spore wall of microsporidia is the initial structure to contact the host cell dir...

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Bibliographic Details
Main Authors: Pengfei Wang, Dufu Li, Qianmin Hai, Siming Liu, Yueyue Zhang, Jun Zhang, Jinshan Xu, Zhengang Ma, Zeyang Zhou
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Microorganisms
Subjects:
<i>Vairimorpha (Nosema) ceranae</i>
spore wall protein
polar tube proteins
subcellular localization
biological functions
Online Access:https://www.mdpi.com/2076-2607/13/1/142
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Summary:<i>Vairimorpha (Nosema) ceranae</i> is a pathogen that affects <i>Apis mellifera</i> and <i>Apis ceranae</i> Fabricius, capable of spreading within and between honeybee colonies. The spore wall of microsporidia is the initial structure to contact the host cell directly, which may play a crucial role in the infection process. Currently, several spore wall proteins have been identified in microsporidia, but only two spore wall proteins from <i>V. ceranae</i> have been characterized. Here, we report the expression and identification of a novel spore wall protein, NcSWP8, with a molecular mass of 21.37 kDa in <i>V. ceranae</i>. Subcellular localization analysis revealed that NcSWP8 was localized on the spore wall of <i>V. ceranae</i>. Co-immunoprecipitation and Far-Western blotting experiments demonstrated that NcSWP8 could stably interact with polar tube proteins, NcPTP2 and NcPTP3. The antibody blocking assay significantly decreased their infection rate, indicating that NcSWP8 played a significant role in the process of <i>V. ceranae</i> infection. These results together suggested that NcSWP8 was a new spore wall protein localized to the spore wall and interacted with the polar tube proteins, playing a crucial role in supporting the formation of the spore wall and potentially affecting the process of infection of <i>V. ceranae</i>.
ISSN:2076-2607

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