Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse Model
C57BL/6 inbred strain mice were instilled intranasally with 150 μg/day of the actinomycete Faeni rectivirgula three days a week as a model of farmer's lung disease. Instilled mice developed a strong alveolitis manifested by a large increase in the number of cells in the bronchoalveolar lavage (...
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| Format: | Article |
| Language: | English |
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Wiley
1992-01-01
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| Series: | Canadian Journal of Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/1992/875050 |
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| _version_ | 1832566172886761472 |
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| author | Michel Denis Marie Bédard Jacques Gagnon Yvon Cormier Michel Laviolette |
| author_facet | Michel Denis Marie Bédard Jacques Gagnon Yvon Cormier Michel Laviolette |
| author_sort | Michel Denis |
| collection | DOAJ |
| description | C57BL/6 inbred strain mice were instilled intranasally with
150 μg/day of the actinomycete Faeni rectivirgula three days a week as a model of farmer's lung disease.
Instilled mice developed a strong alveolitis manifested by a large increase in the number of cells in the
bronchoalveolar lavage (BAL) (4.1 x 104 cells in saline controls versus 5.28x 105 cells in F rectivirgula-instilled
mice). This influx was associated with a substantial release of pro-inflanm1atory cytokine in the BAL; 170
U/mL of interleukin (IL)-1 in instilled mice whereas saline instilled mice had undetectable levels of cytokines
in the BAL. In addition, pulmonary fibrosis was evident in challenged mice at three weeks (twofold increase
in lung hydroxyproline levels). Infusion of a rabbit antimouse tumour necrosis factor-alpha was associated
with an almost complete abrogation of all markers of the disease. Also, administration of cyclosporine A
(50 mg/kg/day) partially prevented the alveolitis (P<0.01) and totally prevented cytokine release and lung
fibrosis in challenged mice. These findings underscore the tmmunological basis of hypersensitivity
pneumonitis, and suggest that antagonism of inflammatory cytokines may hold promise in the treatment
of this pathology. |
| format | Article |
| id | doaj-art-f5f4e68335ef46d19229faffe571b0b9 |
| institution | Kabale University |
| issn | 1180-2332 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases |
| spelling | doaj-art-f5f4e68335ef46d19229faffe571b0b92025-02-03T01:04:54ZengWileyCanadian Journal of Infectious Diseases1180-23321992-01-013Suppl B11111410.1155/1992/875050Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse ModelMichel DenisMarie BédardJacques GagnonYvon CormierMichel LavioletteC57BL/6 inbred strain mice were instilled intranasally with 150 μg/day of the actinomycete Faeni rectivirgula three days a week as a model of farmer's lung disease. Instilled mice developed a strong alveolitis manifested by a large increase in the number of cells in the bronchoalveolar lavage (BAL) (4.1 x 104 cells in saline controls versus 5.28x 105 cells in F rectivirgula-instilled mice). This influx was associated with a substantial release of pro-inflanm1atory cytokine in the BAL; 170 U/mL of interleukin (IL)-1 in instilled mice whereas saline instilled mice had undetectable levels of cytokines in the BAL. In addition, pulmonary fibrosis was evident in challenged mice at three weeks (twofold increase in lung hydroxyproline levels). Infusion of a rabbit antimouse tumour necrosis factor-alpha was associated with an almost complete abrogation of all markers of the disease. Also, administration of cyclosporine A (50 mg/kg/day) partially prevented the alveolitis (P<0.01) and totally prevented cytokine release and lung fibrosis in challenged mice. These findings underscore the tmmunological basis of hypersensitivity pneumonitis, and suggest that antagonism of inflammatory cytokines may hold promise in the treatment of this pathology.http://dx.doi.org/10.1155/1992/875050 |
| spellingShingle | Michel Denis Marie Bédard Jacques Gagnon Yvon Cormier Michel Laviolette Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse Model Canadian Journal of Infectious Diseases |
| title | Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse Model |
| title_full | Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse Model |
| title_fullStr | Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse Model |
| title_full_unstemmed | Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse Model |
| title_short | Involvement of Cytokines in the Pathogenesis of Hypersensitivity Pneumonitis: Evaluation of Immunotherapeutic Measures in a Mouse Model |
| title_sort | involvement of cytokines in the pathogenesis of hypersensitivity pneumonitis evaluation of immunotherapeutic measures in a mouse model |
| url | http://dx.doi.org/10.1155/1992/875050 |
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