Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.)
Plant A/T-rich sequence- and zinc-binding protein (PLATZ) is a type of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. This family is essential for plant growth, development, and stress response. In this study, 15 <i>OsPLATZs</i> were identified in...
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2025-01-01
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| author | Tao Yang Xin-Tong Xu Li-Jun Tang Wen-Tao Wei Yuan-Yuan Zhao Jin-Xin Liu Xue-Feng Yao Heng Zhao Chun-Ming Liu Ai-Ning Bai |
| author_facet | Tao Yang Xin-Tong Xu Li-Jun Tang Wen-Tao Wei Yuan-Yuan Zhao Jin-Xin Liu Xue-Feng Yao Heng Zhao Chun-Ming Liu Ai-Ning Bai |
| author_sort | Tao Yang |
| collection | DOAJ |
| description | Plant A/T-rich sequence- and zinc-binding protein (PLATZ) is a type of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. This family is essential for plant growth, development, and stress response. In this study, 15 <i>OsPLATZs</i> were identified in the rice genome with complete PLATZ-conserved domains by CD-search, similar to those found in angiosperms. Multi-species phylogenetic analysis showed that PLATZs were conserved in photosynthetic organisms, and an evolutionary branch unique to angiosperms was identified among members of the PLATZ family. Fifteen OsPLATZs were represented by five groups, each with distinct characteristics. An analysis of protein structures and sequence motifs showed that OsPLATZs were similar within groups, but varied between them. The expression profile and qRT-PCR results showed that <i>OsPLATZs</i> had distinct expression patterns in different tissues, with some responding to stress induction. Most of the OsPLATZs localized to the nuclei, and were predicted to bind to DNA sequences by AlphaFold3, suggesting that they likely function as conventional transcription factors. We also identified <i>OsPLATZ1</i>, a caryopsis-specific gene that regulates grain filling and caryopsis development in rice. This research lays the foundation for exploring the structural diversity, evolutionary traits, expression profile, and possible roles of PLATZ transcription factors in rice. |
| format | Article |
| id | doaj-art-f54bd25799244c99b3502f97f5919770 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Plants |
| spelling | doaj-art-f54bd25799244c99b3502f97f59197702025-01-24T13:46:31ZengMDPI AGPlants2223-77472025-01-0114215110.3390/plants14020151Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.)Tao Yang0Xin-Tong Xu1Li-Jun Tang2Wen-Tao Wei3Yuan-Yuan Zhao4Jin-Xin Liu5Xue-Feng Yao6Heng Zhao7Chun-Ming Liu8Ai-Ning Bai9Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaPlant A/T-rich sequence- and zinc-binding protein (PLATZ) is a type of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. This family is essential for plant growth, development, and stress response. In this study, 15 <i>OsPLATZs</i> were identified in the rice genome with complete PLATZ-conserved domains by CD-search, similar to those found in angiosperms. Multi-species phylogenetic analysis showed that PLATZs were conserved in photosynthetic organisms, and an evolutionary branch unique to angiosperms was identified among members of the PLATZ family. Fifteen OsPLATZs were represented by five groups, each with distinct characteristics. An analysis of protein structures and sequence motifs showed that OsPLATZs were similar within groups, but varied between them. The expression profile and qRT-PCR results showed that <i>OsPLATZs</i> had distinct expression patterns in different tissues, with some responding to stress induction. Most of the OsPLATZs localized to the nuclei, and were predicted to bind to DNA sequences by AlphaFold3, suggesting that they likely function as conventional transcription factors. We also identified <i>OsPLATZ1</i>, a caryopsis-specific gene that regulates grain filling and caryopsis development in rice. This research lays the foundation for exploring the structural diversity, evolutionary traits, expression profile, and possible roles of PLATZ transcription factors in rice.https://www.mdpi.com/2223-7747/14/2/151PLATZphotosynthetic organismsriceexpression patterncaryopsis development |
| spellingShingle | Tao Yang Xin-Tong Xu Li-Jun Tang Wen-Tao Wei Yuan-Yuan Zhao Jin-Xin Liu Xue-Feng Yao Heng Zhao Chun-Ming Liu Ai-Ning Bai Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.) Plants PLATZ photosynthetic organisms rice expression pattern caryopsis development |
| title | Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.) |
| title_full | Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.) |
| title_fullStr | Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.) |
| title_full_unstemmed | Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.) |
| title_short | Genome-Wide Study of Plant-Specific PLATZ Transcription Factors and Functional Analysis of <i>OsPLATZ1</i> in Regulating Caryopsis Development of Rice (<i>Oryza sativa</i> L.) |
| title_sort | genome wide study of plant specific platz transcription factors and functional analysis of i osplatz1 i in regulating caryopsis development of rice i oryza sativa i l |
| topic | PLATZ photosynthetic organisms rice expression pattern caryopsis development |
| url | https://www.mdpi.com/2223-7747/14/2/151 |
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