<i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae
This study assessed the bioconversion efficiency of <i>Hermetia illucens</i> larvae (BSFL) fed on food waste stored under different conditions, focusing on the nutritional and microbial quality of the resulting larval biomass. Food waste was prepared as a fresh diet (FD) or naturally con...
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2025-01-01
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| author | Zuzana Mašková Juraj Medo Eduard Kolesár Dana Tančinová Eva Ivanišová Dana Urminská Lukáš Hleba Jana Urminská Monika Mrvová Zuzana Barboráková |
| author_facet | Zuzana Mašková Juraj Medo Eduard Kolesár Dana Tančinová Eva Ivanišová Dana Urminská Lukáš Hleba Jana Urminská Monika Mrvová Zuzana Barboráková |
| author_sort | Zuzana Mašková |
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| description | This study assessed the bioconversion efficiency of <i>Hermetia illucens</i> larvae (BSFL) fed on food waste stored under different conditions, focusing on the nutritional and microbial quality of the resulting larval biomass. Food waste was prepared as a fresh diet (FD) or naturally contaminated and stored at 20–22 °C (OS-T, opened storage-tempered) or under refrigeration, at 5–8 °C (CS-C, closed storage-cooled). Refrigerated, closed storage (CS-C) led to the highest rates of waste reduction (91.0%) and bioconversion efficiency (30.2%), with larvae exhibiting the highest protein content (36.83%) compared to the FD (35.5%) and OS-T (34.71%) groups. Microbiome analysis revealed that the CS-C condition promoted beneficial yeasts like <i>Pichia</i> and <i>Diutia</i>, which correlated positively with improved protein content and microbial safety. In contrast, OS-T storage supported spoilage fungi (<i>Mucor</i>, <i>Rhizopus</i>) and elevated total aerobic counts (7.28 log CFU/g), indicating higher microbial risks. The observed trends in waste reduction and protein content most probably relate to differences in microbial profiles, as controlled cooling affected microbial dynamics, preserving substrate quality and supporting larval growth. These findings emphasize the importance of refrigerated, closed storage to optimize bioconversion, improve larval nutritional value, and minimize microbiological hazards. |
| format | Article |
| id | doaj-art-f5377414fc204f2a891aa1338d73bb4d |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-f5377414fc204f2a891aa1338d73bb4d2025-01-24T13:35:51ZengMDPI AGInsects2075-44502025-01-011618710.3390/insects16010087<i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the LarvaeZuzana Mašková0Juraj Medo1Eduard Kolesár2Dana Tančinová3Eva Ivanišová4Dana Urminská5Lukáš Hleba6Jana Urminská7Monika Mrvová8Zuzana Barboráková9Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakiaecol Trade, s.r.o., J. Haška 1, 949 01 Nitra, SlovakiaInstitute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Food Sciences, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Food Sciences, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaThis study assessed the bioconversion efficiency of <i>Hermetia illucens</i> larvae (BSFL) fed on food waste stored under different conditions, focusing on the nutritional and microbial quality of the resulting larval biomass. Food waste was prepared as a fresh diet (FD) or naturally contaminated and stored at 20–22 °C (OS-T, opened storage-tempered) or under refrigeration, at 5–8 °C (CS-C, closed storage-cooled). Refrigerated, closed storage (CS-C) led to the highest rates of waste reduction (91.0%) and bioconversion efficiency (30.2%), with larvae exhibiting the highest protein content (36.83%) compared to the FD (35.5%) and OS-T (34.71%) groups. Microbiome analysis revealed that the CS-C condition promoted beneficial yeasts like <i>Pichia</i> and <i>Diutia</i>, which correlated positively with improved protein content and microbial safety. In contrast, OS-T storage supported spoilage fungi (<i>Mucor</i>, <i>Rhizopus</i>) and elevated total aerobic counts (7.28 log CFU/g), indicating higher microbial risks. The observed trends in waste reduction and protein content most probably relate to differences in microbial profiles, as controlled cooling affected microbial dynamics, preserving substrate quality and supporting larval growth. These findings emphasize the importance of refrigerated, closed storage to optimize bioconversion, improve larval nutritional value, and minimize microbiological hazards.https://www.mdpi.com/2075-4450/16/1/87bioconversionblack soldier flyBSFLfood waste storage<i>Hermetia illucens</i>microbiological safety |
| spellingShingle | Zuzana Mašková Juraj Medo Eduard Kolesár Dana Tančinová Eva Ivanišová Dana Urminská Lukáš Hleba Jana Urminská Monika Mrvová Zuzana Barboráková <i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae Insects bioconversion black soldier fly BSFL food waste storage <i>Hermetia illucens</i> microbiological safety |
| title | <i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae |
| title_full | <i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae |
| title_fullStr | <i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae |
| title_full_unstemmed | <i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae |
| title_short | <i>Hermetia illucens</i> in the Process of Kitchen Waste Biodegradation: The Effect of Different Approaches to Waste Storage on the Microbiological Profile and Nutritional Parameters of the Larvae |
| title_sort | i hermetia illucens i in the process of kitchen waste biodegradation the effect of different approaches to waste storage on the microbiological profile and nutritional parameters of the larvae |
| topic | bioconversion black soldier fly BSFL food waste storage <i>Hermetia illucens</i> microbiological safety |
| url | https://www.mdpi.com/2075-4450/16/1/87 |
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