Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLC
Traditional Chinese medicine (TCM) formulas have a significant clinical efficacy, and the fingerprint technology has been widely accepted to fully reveal the quality of TCM. Whereas, it is a great challenge to establish the fingerprint chromatogram which can fully reflect every single herb material...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/8858501 |
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| _version_ | 1832555250630787072 |
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| author | Hui Jiang Yuansheng Xiao Xingya Xue Hongli Jin Yang Xiang Yanfang Liu Gaowa Jin |
| author_facet | Hui Jiang Yuansheng Xiao Xingya Xue Hongli Jin Yang Xiang Yanfang Liu Gaowa Jin |
| author_sort | Hui Jiang |
| collection | DOAJ |
| description | Traditional Chinese medicine (TCM) formulas have a significant clinical efficacy, and the fingerprint technology has been widely accepted to fully reveal the quality of TCM. Whereas, it is a great challenge to establish the fingerprint chromatogram which can fully reflect every single herb material in a short time. In this study, we used Xiaojin capsule (XJC) as a case and developed a rapid fingerprint method based on increasing the column temperature and flow rate simultaneously combined with computer-aided. First, the elution gradient was optimized based on the retention parameters and peak shape parameters of the four linear gradients, and then, the column temperature and flow rate were increased simultaneously to shorten the analysis time. Next, the standard fingerprint chromatogram of XJC, which can reflect every herb material, was generated. Finally, quality markers were screened through unsupervised cluster analysis and supervised orthogonal partial least squares discrimination analysis. Combining computer-aided with increasing column temperature and flow rate simultaneously can develop the rapid method for establishing HPLC fingerprint of XJC, which can fully reflect every single herb material and provide comprehensive quality control. The strategy for establishing HPLC fingerprint of TCM formula could be applied to other traditional Chinese medicine formulas and herbal medicine. |
| format | Article |
| id | doaj-art-f3f21f51c7144a0fb32de1454bb4a163 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-f3f21f51c7144a0fb32de1454bb4a1632025-02-03T05:49:16ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792021-01-01202110.1155/2021/88585018858501Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLCHui Jiang0Yuansheng Xiao1Xingya Xue2Hongli Jin3Yang Xiang4Yanfang Liu5Gaowa Jin6Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaKey Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaKey Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaKey Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaJianmin Pharmaceutical Group Co., Ltd., Wuhan, Hubei 430000, ChinaKey Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaKey Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaTraditional Chinese medicine (TCM) formulas have a significant clinical efficacy, and the fingerprint technology has been widely accepted to fully reveal the quality of TCM. Whereas, it is a great challenge to establish the fingerprint chromatogram which can fully reflect every single herb material in a short time. In this study, we used Xiaojin capsule (XJC) as a case and developed a rapid fingerprint method based on increasing the column temperature and flow rate simultaneously combined with computer-aided. First, the elution gradient was optimized based on the retention parameters and peak shape parameters of the four linear gradients, and then, the column temperature and flow rate were increased simultaneously to shorten the analysis time. Next, the standard fingerprint chromatogram of XJC, which can reflect every herb material, was generated. Finally, quality markers were screened through unsupervised cluster analysis and supervised orthogonal partial least squares discrimination analysis. Combining computer-aided with increasing column temperature and flow rate simultaneously can develop the rapid method for establishing HPLC fingerprint of XJC, which can fully reflect every single herb material and provide comprehensive quality control. The strategy for establishing HPLC fingerprint of TCM formula could be applied to other traditional Chinese medicine formulas and herbal medicine.http://dx.doi.org/10.1155/2021/8858501 |
| spellingShingle | Hui Jiang Yuansheng Xiao Xingya Xue Hongli Jin Yang Xiang Yanfang Liu Gaowa Jin Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLC International Journal of Analytical Chemistry |
| title | Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLC |
| title_full | Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLC |
| title_fullStr | Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLC |
| title_full_unstemmed | Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLC |
| title_short | Computer-Aided Rapid Establishment of Fingerprint of Xiaojin Capsule by HPLC |
| title_sort | computer aided rapid establishment of fingerprint of xiaojin capsule by hplc |
| url | http://dx.doi.org/10.1155/2021/8858501 |
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