DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERA
Test-system based on competitive enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Group A rotavirus in sera collected from livestock of various species (cattle, pigs) was developed. Bovine rotavirus TE87 strain of serogroup A adapted to continuous porcine embryo kidney cell c...
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/335 |
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| author | O. P. Bjadovskaya Ye. A. Bukhon A. A. Nesterov |
| author_facet | O. P. Bjadovskaya Ye. A. Bukhon A. A. Nesterov |
| author_sort | O. P. Bjadovskaya |
| collection | DOAJ |
| description | Test-system based on competitive enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Group A rotavirus in sera collected from livestock of various species (cattle, pigs) was developed. Bovine rotavirus TE87 strain of serogroup A adapted to continuous porcine embryo kidney cell culture was selected as an antigen for ELISA. The said strain has the infectivity titre of 7.0-7.5 lg TCID50/cm3. It is group-specific and the most technologically suitable for production antigen scaling-up. Specificity of prepared components, rotavirus antigen and hyperimmune rabbit serum, was confirmed by real-time PCR and immunoblotting. The development of competitive ELISA-based test-system included selection of optimal blocking buffer composition as well as optimal antigen dilution, specific hyperimmune serum and anti-species conjugate. Admissible optical density values of control sera and cut-off values were determined. The test-system was examined for its specificity and sensitivity as compared to those ones of INGENASA test-kit (Spain) by parallel testing of 203 cattle sera. Performed studies resulted in the development of sensitive (92%) and specific (94%) competitive ELISA enabling detection of antibodies against Group A rotavirus in cattle and porcine sera with 92.6% accuracy. A total of 2,259 cattle and porcine sera submitted from 48 farms located in various Russian Federation regions in 2016-2017 were tested with the developed test-system. Average proportion of Group A rotavirus-positive cattle and pigs was 42% and 84%, respectively. |
| format | Article |
| id | doaj-art-f39d0d4ddd4749658e3a6c897cc8e6e4 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
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| series | Ветеринария сегодня |
| spelling | doaj-art-f39d0d4ddd4749658e3a6c897cc8e6e42025-02-06T09:52:06ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01046366335DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERAO. P. Bjadovskaya0Ye. A. Bukhon1A. A. Nesterov2FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirTest-system based on competitive enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Group A rotavirus in sera collected from livestock of various species (cattle, pigs) was developed. Bovine rotavirus TE87 strain of serogroup A adapted to continuous porcine embryo kidney cell culture was selected as an antigen for ELISA. The said strain has the infectivity titre of 7.0-7.5 lg TCID50/cm3. It is group-specific and the most technologically suitable for production antigen scaling-up. Specificity of prepared components, rotavirus antigen and hyperimmune rabbit serum, was confirmed by real-time PCR and immunoblotting. The development of competitive ELISA-based test-system included selection of optimal blocking buffer composition as well as optimal antigen dilution, specific hyperimmune serum and anti-species conjugate. Admissible optical density values of control sera and cut-off values were determined. The test-system was examined for its specificity and sensitivity as compared to those ones of INGENASA test-kit (Spain) by parallel testing of 203 cattle sera. Performed studies resulted in the development of sensitive (92%) and specific (94%) competitive ELISA enabling detection of antibodies against Group A rotavirus in cattle and porcine sera with 92.6% accuracy. A total of 2,259 cattle and porcine sera submitted from 48 farms located in various Russian Federation regions in 2016-2017 were tested with the developed test-system. Average proportion of Group A rotavirus-positive cattle and pigs was 42% and 84%, respectively.https://veterinary.arriah.ru/jour/article/view/335ротавирускультура клетокиммуноферментный анализсыворотки кровикрупный рогатый скотсвиньиrotaviruscell cultureenzyme-linked immunosorbent assayseralivestockpigs |
| spellingShingle | O. P. Bjadovskaya Ye. A. Bukhon A. A. Nesterov DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERA Ветеринария сегодня ротавирус культура клеток иммуноферментный анализ сыворотки крови крупный рогатый скот свиньи rotavirus cell culture enzyme-linked immunosorbent assay sera livestock pigs |
| title | DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERA |
| title_full | DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERA |
| title_fullStr | DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERA |
| title_full_unstemmed | DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERA |
| title_short | DEVELOPMENT AND USE OF THE COMPETITIVE ELISA-BASED TEST SYSTEM FOR DETECTION OF ANTIBODIES TO GROUP A ROTAVIRUS IN LIVESTOCK SERA |
| title_sort | development and use of the competitive elisa based test system for detection of antibodies to group a rotavirus in livestock sera |
| topic | ротавирус культура клеток иммуноферментный анализ сыворотки крови крупный рогатый скот свиньи rotavirus cell culture enzyme-linked immunosorbent assay sera livestock pigs |
| url | https://veterinary.arriah.ru/jour/article/view/335 |
| work_keys_str_mv | AT opbjadovskaya developmentanduseofthecompetitiveelisabasedtestsystemfordetectionofantibodiestogrouparotavirusinlivestocksera AT yeabukhon developmentanduseofthecompetitiveelisabasedtestsystemfordetectionofantibodiestogrouparotavirusinlivestocksera AT aanesterov developmentanduseofthecompetitiveelisabasedtestsystemfordetectionofantibodiestogrouparotavirusinlivestocksera |