RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>)
The yellow catfish (<i>Pelteobagrus fulvidraco</i>) industry has expanded to a certain scale and is an important part of aquaculture in China, but frequent diseases have caused huge economic losses. Comprehending the fish’s immune mechanisms, particularly the regulation of the interferon...
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MDPI AG
2024-12-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/10/1/6 |
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| author | Kejun Liu Jiayang Huang Yuting Gui Qian Li Lei Zhang Shuting Xiong |
| author_facet | Kejun Liu Jiayang Huang Yuting Gui Qian Li Lei Zhang Shuting Xiong |
| author_sort | Kejun Liu |
| collection | DOAJ |
| description | The yellow catfish (<i>Pelteobagrus fulvidraco</i>) industry has expanded to a certain scale and is an important part of aquaculture in China, but frequent diseases have caused huge economic losses. Comprehending the fish’s immune mechanisms, particularly the regulation of the interferon (IFN) response, is of paramount importance for future drug development and disease-resistant molecular breeding. Notably, the role of atypical protein kinases, such as the RIO kinase family, in immune regulation is poorly defined. Here, we investigated the roles of yellow catfish RIO kinases, PfRIOK1 and PfRIOK2, in modulating the IFN response through their interaction with PfTBK1, a key player in the RLR signaling pathway. Mechanically, PfRIOK1 and PfRIOK2 negatively regulate the IFN response by interacting with the RIO domains to target and degrade PfTBK1. Our findings reveal that the overexpression of PfRIOK1 and PfRIOK2 led to the decreased expression of IFN-related genes and enhanced viral replication in vitro. Additionally, PfRIOK1 and PfRIOK2 could inhibit PfTBK1-mediated antiviral responses in infected cells. These results suggest that PfRIOK1 and PfRIOK2 act as negative regulators of the IFN response in yellow catfish, providing new insights into the regulatory mechanisms of fish innate immunity and offering target molecules for molecular design breeding in aquaculture. |
| format | Article |
| id | doaj-art-f3685d9fc1684005ac7640cdf94714f4 |
| institution | Kabale University |
| issn | 2410-3888 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj-art-f3685d9fc1684005ac7640cdf94714f42025-01-24T13:32:24ZengMDPI AGFishes2410-38882024-12-01101610.3390/fishes10010006RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>)Kejun Liu0Jiayang Huang1Yuting Gui2Qian Li3Lei Zhang4Shuting Xiong5College of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, ChinaFisheries College, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture and Forestry Science, Hunan Applied Technology University, Changde 415000, ChinaCollege of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, ChinaFisheries College, Hunan Agricultural University, Changsha 410128, ChinaFisheries College, Hunan Agricultural University, Changsha 410128, ChinaThe yellow catfish (<i>Pelteobagrus fulvidraco</i>) industry has expanded to a certain scale and is an important part of aquaculture in China, but frequent diseases have caused huge economic losses. Comprehending the fish’s immune mechanisms, particularly the regulation of the interferon (IFN) response, is of paramount importance for future drug development and disease-resistant molecular breeding. Notably, the role of atypical protein kinases, such as the RIO kinase family, in immune regulation is poorly defined. Here, we investigated the roles of yellow catfish RIO kinases, PfRIOK1 and PfRIOK2, in modulating the IFN response through their interaction with PfTBK1, a key player in the RLR signaling pathway. Mechanically, PfRIOK1 and PfRIOK2 negatively regulate the IFN response by interacting with the RIO domains to target and degrade PfTBK1. Our findings reveal that the overexpression of PfRIOK1 and PfRIOK2 led to the decreased expression of IFN-related genes and enhanced viral replication in vitro. Additionally, PfRIOK1 and PfRIOK2 could inhibit PfTBK1-mediated antiviral responses in infected cells. These results suggest that PfRIOK1 and PfRIOK2 act as negative regulators of the IFN response in yellow catfish, providing new insights into the regulatory mechanisms of fish innate immunity and offering target molecules for molecular design breeding in aquaculture.https://www.mdpi.com/2410-3888/10/1/6<i>Pelteobagrus fulvidraco</i>RIOK1RIOK12TBK1negative regulationantiviral response |
| spellingShingle | Kejun Liu Jiayang Huang Yuting Gui Qian Li Lei Zhang Shuting Xiong RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Fishes <i>Pelteobagrus fulvidraco</i> RIOK1 RIOK12 TBK1 negative regulation antiviral response |
| title | RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) |
| title_full | RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) |
| title_fullStr | RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) |
| title_full_unstemmed | RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) |
| title_short | RIOK1/2 Negatively Regulates the Antiviral Response by Targeting TBK1 in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) |
| title_sort | riok1 2 negatively regulates the antiviral response by targeting tbk1 in yellow catfish i pelteobagrus fulvidraco i |
| topic | <i>Pelteobagrus fulvidraco</i> RIOK1 RIOK12 TBK1 negative regulation antiviral response |
| url | https://www.mdpi.com/2410-3888/10/1/6 |
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