Detection of Protease Activity Using a Fluorescence-Enhancement Globular Substrate
Bovine serum albumin (BSA) highly derivatized with fluorescein isothiocyanate (FITC, isomer I) served as a fluorescent enhancement substrate to measure protease activity. In the native globular BSA structure, the fluorescence of the lysine-conjugated fluorescein moieties was quenched 98%. Proteolyti...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96202rr06 |
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