CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis
Cellular retinaldehyde binding protein (CRALBP) is an autoantigen in spontaneous equine recurrent uveitis. In order to test whether CRALBP contributes to human autoimmune uveitis, the specificity of antibodies from human uveitis patient's sera was first evaluated in two-dimensional (2D) Western...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2007/39245 |
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| author | Cornelia A. Deeg Albert J. Raith Barbara Amann John W. Crabb Stephan R. Thurau Stefanie M. Hauck Marius Ueffing Gerhild Wildner Manfred Stangassinger |
| author_facet | Cornelia A. Deeg Albert J. Raith Barbara Amann John W. Crabb Stephan R. Thurau Stefanie M. Hauck Marius Ueffing Gerhild Wildner Manfred Stangassinger |
| author_sort | Cornelia A. Deeg |
| collection | DOAJ |
| description | Cellular retinaldehyde binding protein (CRALBP) is an autoantigen in spontaneous equine recurrent uveitis. In
order to test whether CRALBP contributes to human autoimmune uveitis, the specificity of antibodies from human
uveitis patient's sera was first evaluated in two-dimensional (2D) Western blot analysis. Subsequent identification of the immunoreactive proteins by mass spectrometry resulted in the identification of CRALBP as a putative autoantigen. Additionally, sera from human uveitis and control patients were by Western blot using purified human recombinant CRALBP. Anti-CRALBP autoantibodies occur more frequently (P<.01) in human uveitis patients than in normal controls. Thirty out of 56 tested uveitis patient's sera contained autoantibodies reactive against CRALBP, compared to only four out of 23 normal control subjects. The presence of CRALBP autoantibodies in 54% of tested uveitis patients supports CRALBP as a possible autoantigen in human autoimmune uveitis. |
| format | Article |
| id | doaj-art-f26d3cc46a6048d3b379760d184ae32f |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-f26d3cc46a6048d3b379760d184ae32f2025-02-03T07:25:26ZengWileyClinical and Developmental Immunology1740-25221740-25302007-01-01200710.1155/2007/3924539245CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune UveitisCornelia A. Deeg0Albert J. Raith1Barbara Amann2John W. Crabb3Stephan R. Thurau4Stefanie M. Hauck5Marius Ueffing6Gerhild Wildner7Manfred Stangassinger8Institute for Animal Physiology, Ludwig-Maximilians University, Veterinärstraße 13, Munich 80539, GermanyInstitute for Animal Physiology, Ludwig-Maximilians University, Veterinärstraße 13, Munich 80539, GermanyInstitute for Animal Physiology, Ludwig-Maximilians University, Veterinärstraße 13, Munich 80539, GermanyCole Eye Institute and Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USASection of Immunobiology, Department of Ophthalmology, Ludwig-Maximilians University, Mathildenstraße 8, Munich 80336, GermanyInstitute of Human Genetics, GSF National-Research Center for Environment and Health, Ingolstaedter Landstraße 1, Neuherberg 85764, GermanyInstitute of Human Genetics, GSF National-Research Center for Environment and Health, Ingolstaedter Landstraße 1, Neuherberg 85764, GermanySection of Immunobiology, Department of Ophthalmology, Ludwig-Maximilians University, Mathildenstraße 8, Munich 80336, GermanyInstitute for Animal Physiology, Ludwig-Maximilians University, Veterinärstraße 13, Munich 80539, GermanyCellular retinaldehyde binding protein (CRALBP) is an autoantigen in spontaneous equine recurrent uveitis. In order to test whether CRALBP contributes to human autoimmune uveitis, the specificity of antibodies from human uveitis patient's sera was first evaluated in two-dimensional (2D) Western blot analysis. Subsequent identification of the immunoreactive proteins by mass spectrometry resulted in the identification of CRALBP as a putative autoantigen. Additionally, sera from human uveitis and control patients were by Western blot using purified human recombinant CRALBP. Anti-CRALBP autoantibodies occur more frequently (P<.01) in human uveitis patients than in normal controls. Thirty out of 56 tested uveitis patient's sera contained autoantibodies reactive against CRALBP, compared to only four out of 23 normal control subjects. The presence of CRALBP autoantibodies in 54% of tested uveitis patients supports CRALBP as a possible autoantigen in human autoimmune uveitis.http://dx.doi.org/10.1155/2007/39245 |
| spellingShingle | Cornelia A. Deeg Albert J. Raith Barbara Amann John W. Crabb Stephan R. Thurau Stefanie M. Hauck Marius Ueffing Gerhild Wildner Manfred Stangassinger CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis Clinical and Developmental Immunology |
| title | CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis |
| title_full | CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis |
| title_fullStr | CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis |
| title_full_unstemmed | CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis |
| title_short | CRALBP is a Highly Prevalent Autoantigen for Human Autoimmune Uveitis |
| title_sort | cralbp is a highly prevalent autoantigen for human autoimmune uveitis |
| url | http://dx.doi.org/10.1155/2007/39245 |
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