Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don
The gesneriaceous perennial plant, Lysionotus serratus, has been used in traditional Chinese medicine. It also has a great development potential as an ornamental plant with its attractive foliage and beautiful flowers. An efficient propagation and regeneration system via direct shoot organogenesis f...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/280384 |
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| author | Qiansheng Li Min Deng Jie Zhang Wei Zhao Yigang Song Quanjian Li Qingjun Huang |
| author_facet | Qiansheng Li Min Deng Jie Zhang Wei Zhao Yigang Song Quanjian Li Qingjun Huang |
| author_sort | Qiansheng Li |
| collection | DOAJ |
| description | The gesneriaceous perennial plant, Lysionotus serratus, has been used in traditional Chinese medicine. It also has a great development potential as an ornamental plant with its attractive foliage and beautiful flowers. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant was established in this study. High active cytokinin (6-benzyladenine (BA) or thidiazuron (TDZ)) was effective for direct organogenesis of initial induction. Murashige and Skoog (MS) growth media containing 0.5 mg L−1 BA alone or with combination of 0.1 mg L−1 α-Naphthaleneacetic acid (NAA) were the most effective for shoot proliferation. High BA concentration (1.0 mg L−1) in the media caused high percentage of vitrified shoots though they introduced high shoot proliferation rate. Histological observation indicated that adventitious shoot regeneration on the medium containing 0.5 mg L−1 BA alone occurred directly from leaf epidermal cells without callus formation. Regenerated shoots rooted well on medium containing half-strength MS medium with 0.5 mg L−1 indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA), and the plantlets successfully acclimatized and grew vigorously in the greenhouse with a 94.2% and 92.1% survival rate. |
| format | Article |
| id | doaj-art-f1e8afb11ef246899c61185c71b65d8d |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f1e8afb11ef246899c61185c71b65d8d2025-02-03T07:25:02ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/280384280384Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. DonQiansheng Li0Min Deng1Jie Zhang2Wei Zhao3Yigang Song4Quanjian Li5Qingjun Huang6School of Ecology, Shanghai Institute of Technology, 100 Haiquan Road, Fengxian, Shanghai 201418, ChinaShanghai Chenshan Plant Sciences Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaGenetic Diagnosis Center, Yunnan Provincial Key Laboratory for Birth Defects and Genetic Diseases, The First People’s Hospital of Kunming, Yunnan Province 650032, ChinaShanghai Chenshan Plant Sciences Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaShanghai Chenshan Plant Sciences Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaShanghai Chenshan Plant Sciences Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaSchool of Ecology, Shanghai Institute of Technology, 100 Haiquan Road, Fengxian, Shanghai 201418, ChinaThe gesneriaceous perennial plant, Lysionotus serratus, has been used in traditional Chinese medicine. It also has a great development potential as an ornamental plant with its attractive foliage and beautiful flowers. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant was established in this study. High active cytokinin (6-benzyladenine (BA) or thidiazuron (TDZ)) was effective for direct organogenesis of initial induction. Murashige and Skoog (MS) growth media containing 0.5 mg L−1 BA alone or with combination of 0.1 mg L−1 α-Naphthaleneacetic acid (NAA) were the most effective for shoot proliferation. High BA concentration (1.0 mg L−1) in the media caused high percentage of vitrified shoots though they introduced high shoot proliferation rate. Histological observation indicated that adventitious shoot regeneration on the medium containing 0.5 mg L−1 BA alone occurred directly from leaf epidermal cells without callus formation. Regenerated shoots rooted well on medium containing half-strength MS medium with 0.5 mg L−1 indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA), and the plantlets successfully acclimatized and grew vigorously in the greenhouse with a 94.2% and 92.1% survival rate.http://dx.doi.org/10.1155/2013/280384 |
| spellingShingle | Qiansheng Li Min Deng Jie Zhang Wei Zhao Yigang Song Quanjian Li Qingjun Huang Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don The Scientific World Journal |
| title | Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don |
| title_full | Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don |
| title_fullStr | Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don |
| title_full_unstemmed | Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don |
| title_short | Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don |
| title_sort | shoot organogenesis and plant regeneration from leaf explants of lysionotus serratus d don |
| url | http://dx.doi.org/10.1155/2013/280384 |
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