Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer
We determined that human head and neck cancer cells (HSC-3 cell line) contain a subpopulation displaying cancer stem cell (CSC) properties and are very tumorigenic. Specifically, we investigated whether different sizes of hyaluronan (HA) (e.g., 5 kDa, 20 kDa, 200 kDa, or 700 kDa-HA-sizes) play a rol...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2015/989070 |
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| author | Marisa Shiina Lilly Y. W. Bourguignon |
| author_facet | Marisa Shiina Lilly Y. W. Bourguignon |
| author_sort | Marisa Shiina |
| collection | DOAJ |
| description | We determined that human head and neck cancer cells (HSC-3 cell line) contain a subpopulation displaying cancer stem cell (CSC) properties and are very tumorigenic. Specifically, we investigated whether different sizes of hyaluronan (HA) (e.g., 5 kDa, 20 kDa, 200 kDa, or 700 kDa-HA-sizes) play a role in regulating these CSCs. First, we observed that 200 kDa-HA (but not other sizes of HA) preferentially induces certain stem cell marker expression resulting in self-renewal and clonal formation of these cells. Further analyses indicate that 200 kDa-HA selectively stimulates the expression of a panel of microRNAs (most noticeably miR-10b) in these CSCs. Survival protein (cIAP-1) expression was also stimulated by 200 kDa-HA in these CSCs leading to cisplatin resistance. Furthermore, our results indicate that the anti-miR-10 inhibitor not only decreases survival protein expression, but also increases chemosensitivity of the 200 kDa-HA-treated CSCs. These findings strongly support the contention that 200 kDa-HA plays a pivotal role in miR-10 production leading to survival protein upregulation and chemoresistance in CSCs. Together, our findings suggest that selective activation of oncogenic signaling by certain sizes of HA (e.g., 200 kDa-HA) may be instrumental in the formation of CSC functions leading to tumor cell survival and chemoresistance in head and neck cancer progression. |
| format | Article |
| id | doaj-art-f0ee6231efd44eafafac80cce0ed1fc6 |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-f0ee6231efd44eafafac80cce0ed1fc62025-02-03T07:25:49ZengWileyInternational Journal of Cell Biology1687-88761687-88842015-01-01201510.1155/2015/989070989070Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck CancerMarisa Shiina0Lilly Y. W. Bourguignon1San Francisco Veterans Affairs Medical Center and Department of Medicine, University of California at San Francisco and Endocrine Unit (111N2), 4150 Clement Street, San Francisco, CA 94121, USASan Francisco Veterans Affairs Medical Center and Department of Medicine, University of California at San Francisco and Endocrine Unit (111N2), 4150 Clement Street, San Francisco, CA 94121, USAWe determined that human head and neck cancer cells (HSC-3 cell line) contain a subpopulation displaying cancer stem cell (CSC) properties and are very tumorigenic. Specifically, we investigated whether different sizes of hyaluronan (HA) (e.g., 5 kDa, 20 kDa, 200 kDa, or 700 kDa-HA-sizes) play a role in regulating these CSCs. First, we observed that 200 kDa-HA (but not other sizes of HA) preferentially induces certain stem cell marker expression resulting in self-renewal and clonal formation of these cells. Further analyses indicate that 200 kDa-HA selectively stimulates the expression of a panel of microRNAs (most noticeably miR-10b) in these CSCs. Survival protein (cIAP-1) expression was also stimulated by 200 kDa-HA in these CSCs leading to cisplatin resistance. Furthermore, our results indicate that the anti-miR-10 inhibitor not only decreases survival protein expression, but also increases chemosensitivity of the 200 kDa-HA-treated CSCs. These findings strongly support the contention that 200 kDa-HA plays a pivotal role in miR-10 production leading to survival protein upregulation and chemoresistance in CSCs. Together, our findings suggest that selective activation of oncogenic signaling by certain sizes of HA (e.g., 200 kDa-HA) may be instrumental in the formation of CSC functions leading to tumor cell survival and chemoresistance in head and neck cancer progression.http://dx.doi.org/10.1155/2015/989070 |
| spellingShingle | Marisa Shiina Lilly Y. W. Bourguignon Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer International Journal of Cell Biology |
| title | Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer |
| title_full | Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer |
| title_fullStr | Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer |
| title_full_unstemmed | Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer |
| title_short | Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer |
| title_sort | selective activation of cancer stem cells by size specific hyaluronan in head and neck cancer |
| url | http://dx.doi.org/10.1155/2015/989070 |
| work_keys_str_mv | AT marisashiina selectiveactivationofcancerstemcellsbysizespecifichyaluronaninheadandneckcancer AT lillyywbourguignon selectiveactivationofcancerstemcellsbysizespecifichyaluronaninheadandneckcancer |