Detection of Staphylococcus Enterotoxin sea and seb in Milk Samples by Duplex Droplet Digital PCR

Detection of Staphylococcus Enterotoxin sea and seb in Milk Samples by Duplex Droplet Digital PCR

This study sought to develop a method to accurately and quantitatively measure the staphylococcal enterotoxin genes sea and seb in milk samples using duplex droplet digital polymerase chain reaction (ddPCR). Specific primers and probes were designed for sea and seb. By optimizing the concentrations...

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Bibliographic Details
Main Authors: Xiaoyue Wei, Wenzhou Wang, Tong Wang, Yuhua Yang, Yahui Guo, Lijin Long, Jiaming Fan, Fanliang Meng, Wentao Liu, Wanting Wang, Yakun Zhao, Jianling Chen, Fei Zhao, Jianzhong Zhang, Xiaomei Yan
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Journal of Food Protection
Subjects:
Duplex ddPCR
Enterotoxin sea and seb
Quantitative detection
Staphylococcus aureus
Online Access:http://www.sciencedirect.com/science/article/pii/S0362028X25001218
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http://www.sciencedirect.com/science/article/pii/S0362028X25001218

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