Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology
The proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhan...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/819793 |
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| author | Péter Bakonyi Nándor Nemestóthy Katalin Bélafi-Bakó |
| author_facet | Péter Bakonyi Nándor Nemestóthy Katalin Bélafi-Bakó |
| author_sort | Péter Bakonyi |
| collection | DOAJ |
| description | The proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhanced hydrogen fermentation capability. In present paper, it was aimed to show the utility both of strain engineering and process optimization through a comparative study of wild-type and genetically modified E. coli strains, where the effect of two major operational factors (substrate concentration and pH) on bioH2 production was investigated by experimental design and response surface methodology (RSM) was used to determine the suitable conditions in order to obtain maximum yields. The results revealed that by employing the genetically engineered E. coli (DJT 135) strain under optimized conditions (pH: 6.5; Formate conc.: 1.25 g/L), 0.63 mol H2/mol formate could be attained, which was 1.5 times higher compared to the wild-type E. coli (XL1-BLUE) that produced 0.42 mol H2/mol formate (pH: 6.4; Formate conc.: 1.3 g/L). |
| format | Article |
| id | doaj-art-ef43f2f5cd644fe3aa4febd0487e2b1c |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ef43f2f5cd644fe3aa4febd0487e2b1c2025-02-03T01:03:48ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/819793819793Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface MethodologyPéter Bakonyi0Nándor Nemestóthy1Katalin Bélafi-Bakó2Research Institute on Bioengineering, Membrane Technology, and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, HungaryResearch Institute on Bioengineering, Membrane Technology, and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, HungaryResearch Institute on Bioengineering, Membrane Technology, and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, HungaryThe proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhanced hydrogen fermentation capability. In present paper, it was aimed to show the utility both of strain engineering and process optimization through a comparative study of wild-type and genetically modified E. coli strains, where the effect of two major operational factors (substrate concentration and pH) on bioH2 production was investigated by experimental design and response surface methodology (RSM) was used to determine the suitable conditions in order to obtain maximum yields. The results revealed that by employing the genetically engineered E. coli (DJT 135) strain under optimized conditions (pH: 6.5; Formate conc.: 1.25 g/L), 0.63 mol H2/mol formate could be attained, which was 1.5 times higher compared to the wild-type E. coli (XL1-BLUE) that produced 0.42 mol H2/mol formate (pH: 6.4; Formate conc.: 1.3 g/L).http://dx.doi.org/10.1100/2012/819793 |
| spellingShingle | Péter Bakonyi Nándor Nemestóthy Katalin Bélafi-Bakó Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology The Scientific World Journal |
| title | Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology |
| title_full | Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology |
| title_fullStr | Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology |
| title_full_unstemmed | Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology |
| title_short | Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology |
| title_sort | comparative study of various e coli strains for biohydrogen production applying response surface methodology |
| url | http://dx.doi.org/10.1100/2012/819793 |
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