Advances in adipose-derived stem cell harvesting: tissue blocks vs. lipoaspirates
Ensuring a nonenzymatic isolation method for human adipose-derived mesenchymal stem cells (hADSCs) is crucial for safe therapeutic applications. In this study, we introduced an improved mechanical technique to isolate hADSCs from subcutaneous adipose tissue. A total of 382.42 g of adipose tissue was...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
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| Series: | Journal of Taibah University for Science |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2025.2483594 |
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| Summary: | Ensuring a nonenzymatic isolation method for human adipose-derived mesenchymal stem cells (hADSCs) is crucial for safe therapeutic applications. In this study, we introduced an improved mechanical technique to isolate hADSCs from subcutaneous adipose tissue. A total of 382.42 g of adipose tissue was obtained from 19 consented donors, including lipoaspirates (mean: 16.98 g, n = 9) and tissue blocks (mean: 12.11 g, n = 19). Following washing, mincing, and centrifugation, stromal vascular fraction (SVF) cells were collected and subsequently cultured. Notably, the viable SVF yield was 12.47 × 104 cells/ml from tissue blocks and 10.73 × 104 cells/ml from lipoaspirates. Furthermore, at passage 2, hADSCs exhibited typical MSC markers (CD90+/CD73+/CD29+/CD105+/CD34−), while at passage 5, they successfully differentiated into adipocytes, osteoblasts, and chondrocytes. Overall, this method effectively isolates and expands hADSCs without xenogeneic contamination, thereby providing a practical and safe approach for clinical applications. |
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| ISSN: | 1658-3655 |