Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables
Rapid detection of target foodborne pathogens plays more and more significant roles in food safety, which requires the efficiency, sensitivity, and accuracy. In this research, we proposed a new strategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of S...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002610 |
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| _version_ | 1832569087746637824 |
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| author | Bin Li Hanling Wang Jianguo Xu Wei Qu Li Yao Bangben Yao Chao Yan Wei Chen |
| author_facet | Bin Li Hanling Wang Jianguo Xu Wei Qu Li Yao Bangben Yao Chao Yan Wei Chen |
| author_sort | Bin Li |
| collection | DOAJ |
| description | Rapid detection of target foodborne pathogens plays more and more significant roles in food safety, which requires the efficiency, sensitivity, and accuracy. In this research, we proposed a new strategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of Salmonella without traditional enrichment-culture. The designed syringe-assisted-filtration can contribute to simultaneous collection and concentration of target bacterium from vegetable samples in just 3 min, resolving the drawbacks of traditional random sampling protocols. After simple and convenient ultrasonication, samples can be directly amplified at 39 °C in 25 min and the amplicons are qualitatively and quantitatively analyzed with the designed lateral-flow-strip in 5 min. Finally, satisfied results have been achieved within 40 min, which greatly improve the efficiency while the accuracy is also guaranteed. Furthermore, all detection steps can be completed under instrument-free conditions. This method will hold great promise for target pathogen detection in the resource-limited district, or for emergency on-site identification. |
| format | Article |
| id | doaj-art-ee2b4ccab8bb423ebf6cae53c1030a32 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-ee2b4ccab8bb423ebf6cae53c1030a322025-02-02T23:25:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112411671173Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetablesBin Li0Hanling Wang1Jianguo Xu2Wei Qu3Li Yao4Bangben Yao5Chao Yan6Wei Chen7Engineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, China; Corresponding authors at: Hefei University of Technology, Hefei 230009, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, China; Anhui Provincial Institute of Product Quality Supervision and Inspection, Hefei 230051, ChinaAnhui Provincial Institute of Product Quality Supervision and Inspection, Hefei 230051, ChinaEngineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei 230009, China; Corresponding authors at: Hefei University of Technology, Hefei 230009, ChinaRapid detection of target foodborne pathogens plays more and more significant roles in food safety, which requires the efficiency, sensitivity, and accuracy. In this research, we proposed a new strategy of isothermal-molecular-amplification integrated with lateral-flow-strip for rapid detection of Salmonella without traditional enrichment-culture. The designed syringe-assisted-filtration can contribute to simultaneous collection and concentration of target bacterium from vegetable samples in just 3 min, resolving the drawbacks of traditional random sampling protocols. After simple and convenient ultrasonication, samples can be directly amplified at 39 °C in 25 min and the amplicons are qualitatively and quantitatively analyzed with the designed lateral-flow-strip in 5 min. Finally, satisfied results have been achieved within 40 min, which greatly improve the efficiency while the accuracy is also guaranteed. Furthermore, all detection steps can be completed under instrument-free conditions. This method will hold great promise for target pathogen detection in the resource-limited district, or for emergency on-site identification.http://www.sciencedirect.com/science/article/pii/S2213453022002610VegetablesSalmonellaFiltration enrichmentCulture-free detectionEnzymatic recombinase amplification (ERA)Lateral flow strip (LFS) |
| spellingShingle | Bin Li Hanling Wang Jianguo Xu Wei Qu Li Yao Bangben Yao Chao Yan Wei Chen Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables Food Science and Human Wellness Vegetables Salmonella Filtration enrichment Culture-free detection Enzymatic recombinase amplification (ERA) Lateral flow strip (LFS) |
| title | Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables |
| title_full | Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables |
| title_fullStr | Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables |
| title_full_unstemmed | Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables |
| title_short | Filtration assisted pretreatment for rapid enrichment and accurate detection of Salmonella in vegetables |
| title_sort | filtration assisted pretreatment for rapid enrichment and accurate detection of salmonella in vegetables |
| topic | Vegetables Salmonella Filtration enrichment Culture-free detection Enzymatic recombinase amplification (ERA) Lateral flow strip (LFS) |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002610 |
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