A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells
Abstract Extracellular vesicles (EVs) are not only involved in cell-to-cell communications but have other functions as “garbage bags”, as bringing nutrients to cells, and as inducing mineral during bone formation and ectopic calcification. These minuscule entities significantly contribute to the reg...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-87371-3 |
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| author | Yingying Tong Jie Sun Xin Jiang Xu Jia Huimin Xiao Hua Wang Guanghua Yang |
| author_facet | Yingying Tong Jie Sun Xin Jiang Xu Jia Huimin Xiao Hua Wang Guanghua Yang |
| author_sort | Yingying Tong |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) are not only involved in cell-to-cell communications but have other functions as “garbage bags”, as bringing nutrients to cells, and as inducing mineral during bone formation and ectopic calcification. These minuscule entities significantly contribute to the regulation of bodily functions. However, the clinical application of EVs faces challenges due to limited production yield and targeting efficiency. In our study, we propose a method for efficiently harvesting EVs utilizing simian virus 40 large T antigen (SV40LT) immortalized human placental chorionic mesenchymal stromal cells (CMSCs). We investigated immortalized placental chorionic mesenchymal stromal cells (imCMSCs), a stromal cell line that surpasses the growth limitations of primary passage cells while retaining phenotypic characteristics and differentiation potential. This development offers the prospect of a consistent, uniform source of EVs, which is essential for regenerative medicine. Our findings indicate that the immortalization process preserves the particle size, quantity and surface marker profiles of EVs, providing a possible approach to produce high-yield EVs suitable for disease diagnosis and treatment. |
| format | Article |
| id | doaj-art-ee0d6a0c08554cd18da51e0dca58779b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ee0d6a0c08554cd18da51e0dca58779b2025-02-02T12:21:57ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-87371-3A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cellsYingying Tong0Jie Sun1Xin Jiang2Xu Jia3Huimin Xiao4Hua Wang5Guanghua Yang6International Research Center for Biological Sciences, Ministry of Science and Technology, Shanghai Ocean UniversityInternational Research Center for Biological Sciences, Ministry of Science and Technology, Shanghai Ocean UniversityInternational Research Center for Biological Sciences, Ministry of Science and Technology, Shanghai Ocean UniversityInternational Research Center for Biological Sciences, Ministry of Science and Technology, Shanghai Ocean UniversityInternational Research Center for Biological Sciences, Ministry of Science and Technology, Shanghai Ocean UniversityShanghai Telebio Biomedical Technology Co., LTDInternational Research Center for Biological Sciences, Ministry of Science and Technology, Shanghai Ocean UniversityAbstract Extracellular vesicles (EVs) are not only involved in cell-to-cell communications but have other functions as “garbage bags”, as bringing nutrients to cells, and as inducing mineral during bone formation and ectopic calcification. These minuscule entities significantly contribute to the regulation of bodily functions. However, the clinical application of EVs faces challenges due to limited production yield and targeting efficiency. In our study, we propose a method for efficiently harvesting EVs utilizing simian virus 40 large T antigen (SV40LT) immortalized human placental chorionic mesenchymal stromal cells (CMSCs). We investigated immortalized placental chorionic mesenchymal stromal cells (imCMSCs), a stromal cell line that surpasses the growth limitations of primary passage cells while retaining phenotypic characteristics and differentiation potential. This development offers the prospect of a consistent, uniform source of EVs, which is essential for regenerative medicine. Our findings indicate that the immortalization process preserves the particle size, quantity and surface marker profiles of EVs, providing a possible approach to produce high-yield EVs suitable for disease diagnosis and treatment.https://doi.org/10.1038/s41598-025-87371-3PMSCsImmortalitySV40LTExtracellular vesicles |
| spellingShingle | Yingying Tong Jie Sun Xin Jiang Xu Jia Huimin Xiao Hua Wang Guanghua Yang A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells Scientific Reports PMSCs Immortality SV40LT Extracellular vesicles |
| title | A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells |
| title_full | A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells |
| title_fullStr | A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells |
| title_full_unstemmed | A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells |
| title_short | A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells |
| title_sort | study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells |
| topic | PMSCs Immortality SV40LT Extracellular vesicles |
| url | https://doi.org/10.1038/s41598-025-87371-3 |
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