The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice
The influence of an anti-allergic agent, suplatast tosilate (IPD-1151T; (±)-[2-[4-(3-ethoxy–2-hydroxypropoxy)phenyl-carbamoyl]-ethyl] dimethylsulfonium p-toluenesulfonate) on allergic bronchoconstriction induced by allergen and methacholine (MCh) were examined in mice. BALB/c mice were sensitized by...
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/096293500411532 |
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| _version_ | 1832565208112955392 |
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| author | Kazuhito Asano Tetsuya Mizutani Toshikazu Shimane Masataka Hisano Tadashi Hisamitsu Harumi Suzaki |
| author_facet | Kazuhito Asano Tetsuya Mizutani Toshikazu Shimane Masataka Hisano Tadashi Hisamitsu Harumi Suzaki |
| author_sort | Kazuhito Asano |
| collection | DOAJ |
| description | The influence of an anti-allergic agent, suplatast tosilate (IPD-1151T; (±)-[2-[4-(3-ethoxy–2-hydroxypropoxy)phenyl-carbamoyl]-ethyl] dimethylsulfonium p-toluenesulfonate) on allergic bronchoconstriction induced by allergen and methacholine (MCh) were examined in mice. BALB/c mice were sensitized by intraperitoneal injection of dinitrophenylated-keyhole limpet hemocyanin (DNP-KLH) mixed with
Al(OH)3 (DNP-KLH). IPD-1151T was administered orally once a day for either 5 or 14 days in doses of 10, 30 or 100 mg/kg. Bronchoconstriction was measured 24 h after the final drug administration. IPD-1151T inhibited both antigen- and MCh-mediated bronchoconstriction in actively sensitized mice. The inhibition induced was closely related to the dose and frequency of oral administration of the agent. We also examined the effect of IPD-1151T on IgE production in response to DNP-KLH immunization. IPD-1151T inhibited dose-dependently both total and specific IgE concentrations in serum prepared from mice 15 days after im munization. These results strongly indicate that IPD-1151T inhibits IgE
production in vivo and results in attenuating effect on bronchoconstriction. |
| format | Article |
| id | doaj-art-ed8bd14e565a4eee9d91de52ef6cf6ea |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ed8bd14e565a4eee9d91de52ef6cf6ea2025-02-03T01:09:03ZengWileyMediators of Inflammation0962-93511466-18612000-01-0192778410.1080/096293500411532The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized miceKazuhito Asano0Tetsuya Mizutani1Toshikazu Shimane2Masataka Hisano3Tadashi Hisamitsu4Harumi Suzaki5Department of Physiology, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Physiology, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Physiology, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142–8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Shinagawa-ku, Tokyo 142–8555, JapanThe influence of an anti-allergic agent, suplatast tosilate (IPD-1151T; (±)-[2-[4-(3-ethoxy–2-hydroxypropoxy)phenyl-carbamoyl]-ethyl] dimethylsulfonium p-toluenesulfonate) on allergic bronchoconstriction induced by allergen and methacholine (MCh) were examined in mice. BALB/c mice were sensitized by intraperitoneal injection of dinitrophenylated-keyhole limpet hemocyanin (DNP-KLH) mixed with Al(OH)3 (DNP-KLH). IPD-1151T was administered orally once a day for either 5 or 14 days in doses of 10, 30 or 100 mg/kg. Bronchoconstriction was measured 24 h after the final drug administration. IPD-1151T inhibited both antigen- and MCh-mediated bronchoconstriction in actively sensitized mice. The inhibition induced was closely related to the dose and frequency of oral administration of the agent. We also examined the effect of IPD-1151T on IgE production in response to DNP-KLH immunization. IPD-1151T inhibited dose-dependently both total and specific IgE concentrations in serum prepared from mice 15 days after im munization. These results strongly indicate that IPD-1151T inhibits IgE production in vivo and results in attenuating effect on bronchoconstriction.http://dx.doi.org/10.1080/096293500411532 |
| spellingShingle | Kazuhito Asano Tetsuya Mizutani Toshikazu Shimane Masataka Hisano Tadashi Hisamitsu Harumi Suzaki The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice Mediators of Inflammation |
| title | The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice |
| title_full | The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice |
| title_fullStr | The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice |
| title_full_unstemmed | The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice |
| title_short | The inhibitory effect of anti-allergic agent suplatast tosilate (IPD–1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice |
| title_sort | inhibitory effect of anti allergic agent suplatast tosilate ipd 1151t on methacholine and allergen induced bronchoconstriction in sensitized mice |
| url | http://dx.doi.org/10.1080/096293500411532 |
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