Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China
ABSTRACT In poultry, fowl adenovirus (FAdV) and co-infected viruses (such as avian hepatitis E virus, aHEV) are likely to cause decreased egg production, inclusion body hepatitis, and pericardial effusion syndrome. From July to September 2023, eight poultry farms of commercial broilers and commercia...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01338-24 |
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| author | Yuan Zhang Huanyu Xu Yinuo Tian Jirong Tang Huanqing Lin Yani Sun Qin Zhao En-Min Zhou Yiyang Chen Baoyuan Liu |
| author_facet | Yuan Zhang Huanyu Xu Yinuo Tian Jirong Tang Huanqing Lin Yani Sun Qin Zhao En-Min Zhou Yiyang Chen Baoyuan Liu |
| author_sort | Yuan Zhang |
| collection | DOAJ |
| description | ABSTRACT In poultry, fowl adenovirus (FAdV) and co-infected viruses (such as avian hepatitis E virus, aHEV) are likely to cause decreased egg production, inclusion body hepatitis, and pericardial effusion syndrome. From July to September 2023, eight poultry farms of commercial broilers and commercial layers suffered from increased mortality, decreased egg production, and the presence of hydropericardium-hepatitis syndrome-like gross lesions in Shaanxi province, China. To determine the source of the infection, the viruses of aHEV, FAdV, avian leukosis virus (ALV), Marek’s disease virus (MDV), Newcastle disease virus (NDV), and H9N2 avian influenza virus (AIV) were detected. A total of 240 liver samples were collected from eight farms and detected by molecular etiology methods. The samples were tested positive for FAdV and aHEV and negative for NDV, MDV, ALV, and H9N2 AIV. Phylogenetic analysis based on the hexon loop-1 gene showed that these isolates were clustered into three distinct serotypes: FAdV serotype 4 (FAdV-4), FAdV-8a, and FAdV-8b. The partial ORF2 gene sequences obtained in this study shared the highest identity (76%–97%) with corresponding sequences of other known avian HEV isolates and belonged to gene type 3 avian HEV. Meanwhile, the isolated FAdV in this study could multiply in leghorn male hepatoma (LMH) cells. Overall, the disease may be mainly caused by FAdV and aHEV coinfection on these farms, and this is the first study to discover FAdV-4/8a/8b and avian HEV coinfection in a farm in China.IMPORTANCELast year, eight poultry farms of commercial broilers and commercial layers suffered a serious disease in Shaanxi province, China. After molecular etiological investigation, the disease may be mainly caused by fowl adenovirus (FAdV) (FAdV-4, FAdV-8a, and FAdV-8b) and avian hepatitis E virus (aHEV) (genotype 3 avian HEV) coinfection on these flocks, and this is the first study to find FAdV-4/8a/8b and avian HEV coinfection in a farm. It provides a valuable foundation for the prevention and control of FAdV and avian HEV coinfection in chicken farms. |
| format | Article |
| id | doaj-art-ed89fe057cc24ee891df66d218908e7c |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-ed89fe057cc24ee891df66d218908e7c2025-02-04T14:03:41ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.01338-24Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, ChinaYuan Zhang0Huanyu Xu1Yinuo Tian2Jirong Tang3Huanqing Lin4Yani Sun5Qin Zhao6En-Min Zhou7Yiyang Chen8Baoyuan Liu9Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaKongtong Animal Disease Prevention and Control Center, Pingliang, Gansu, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, ChinaABSTRACT In poultry, fowl adenovirus (FAdV) and co-infected viruses (such as avian hepatitis E virus, aHEV) are likely to cause decreased egg production, inclusion body hepatitis, and pericardial effusion syndrome. From July to September 2023, eight poultry farms of commercial broilers and commercial layers suffered from increased mortality, decreased egg production, and the presence of hydropericardium-hepatitis syndrome-like gross lesions in Shaanxi province, China. To determine the source of the infection, the viruses of aHEV, FAdV, avian leukosis virus (ALV), Marek’s disease virus (MDV), Newcastle disease virus (NDV), and H9N2 avian influenza virus (AIV) were detected. A total of 240 liver samples were collected from eight farms and detected by molecular etiology methods. The samples were tested positive for FAdV and aHEV and negative for NDV, MDV, ALV, and H9N2 AIV. Phylogenetic analysis based on the hexon loop-1 gene showed that these isolates were clustered into three distinct serotypes: FAdV serotype 4 (FAdV-4), FAdV-8a, and FAdV-8b. The partial ORF2 gene sequences obtained in this study shared the highest identity (76%–97%) with corresponding sequences of other known avian HEV isolates and belonged to gene type 3 avian HEV. Meanwhile, the isolated FAdV in this study could multiply in leghorn male hepatoma (LMH) cells. Overall, the disease may be mainly caused by FAdV and aHEV coinfection on these farms, and this is the first study to discover FAdV-4/8a/8b and avian HEV coinfection in a farm in China.IMPORTANCELast year, eight poultry farms of commercial broilers and commercial layers suffered a serious disease in Shaanxi province, China. After molecular etiological investigation, the disease may be mainly caused by fowl adenovirus (FAdV) (FAdV-4, FAdV-8a, and FAdV-8b) and avian hepatitis E virus (aHEV) (genotype 3 avian HEV) coinfection on these flocks, and this is the first study to find FAdV-4/8a/8b and avian HEV coinfection in a farm. It provides a valuable foundation for the prevention and control of FAdV and avian HEV coinfection in chicken farms.https://journals.asm.org/doi/10.1128/spectrum.01338-24hepatitis E virusfowl adenoviruscoinfectionepidemiological investigation |
| spellingShingle | Yuan Zhang Huanyu Xu Yinuo Tian Jirong Tang Huanqing Lin Yani Sun Qin Zhao En-Min Zhou Yiyang Chen Baoyuan Liu Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China Microbiology Spectrum hepatitis E virus fowl adenovirus coinfection epidemiological investigation |
| title | Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China |
| title_full | Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China |
| title_fullStr | Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China |
| title_full_unstemmed | Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China |
| title_short | Coinfection of avian hepatitis E virus and different serotypes of fowl adenovirus in chicken flocks in Shaanxi, China |
| title_sort | coinfection of avian hepatitis e virus and different serotypes of fowl adenovirus in chicken flocks in shaanxi china |
| topic | hepatitis E virus fowl adenovirus coinfection epidemiological investigation |
| url | https://journals.asm.org/doi/10.1128/spectrum.01338-24 |
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