VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue
Adipose tissue secrets adipokines and fatty acids, which may contribute to obesity-associated vascular dysfunction and cardiovascular risk. This study investigated which factors are responsible for the synergistic effect of adipokine and oleic acid- (OA-) induced proliferation of human vascular smoo...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/982458 |
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| author | Raphaela Schlich Miriam Willems Sabrina Greulich Florian Ruppe Wolfram Trudo Knoefel D. Margriet Ouwens Bujar Maxhera Artur Lichtenberg Jürgen Eckel Henrike Sell |
| author_facet | Raphaela Schlich Miriam Willems Sabrina Greulich Florian Ruppe Wolfram Trudo Knoefel D. Margriet Ouwens Bujar Maxhera Artur Lichtenberg Jürgen Eckel Henrike Sell |
| author_sort | Raphaela Schlich |
| collection | DOAJ |
| description | Adipose tissue secrets adipokines and fatty acids, which may contribute to obesity-associated vascular dysfunction and cardiovascular risk. This study investigated which factors are responsible for the synergistic effect of adipokine and oleic acid- (OA-) induced proliferation of human vascular smooth muscle cells (VSMC). Adipocyte-conditioned medium (CM) from human adipocytes induces proliferation of VSMC in correlation to its vascular endothelial growth factor (VEGF) content. CM increases VEGF-receptor (VEGF-R) 1 and 2 expression and VEGF secretion of VSMC, while OA only stimulates VEGF secretion. VEGF neutralization abrogates CM- and OA-induced proliferation and considerably reduces proliferation induced by CM and OA in combination. VEGF release is higher from visceral adipose tissue (VAT) of obese subjects compared to subcutaneous adipose tissue (SAT) and VAT from lean controls. Furthermore, VEGF release from VAT correlates with its proliferative effect. Perivascular adipose tissue (PAT) from type 2 diabetic patients releases significantly higher amounts of VEGF and induces stronger proliferation of VSMC as compared to SAT and SAT/PAT of nondiabetics. In conclusion, VEGF is mediating CM-induced proliferation of VSMC. As this adipokine is released in high amounts from VAT of obese patients and PAT of diabetic patients, VEGF might link adipose tissue inflammation to increased VSMC proliferation. |
| format | Article |
| id | doaj-art-ed89bd1b662b4ccca2b0a313715b231f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ed89bd1b662b4ccca2b0a313715b231f2025-02-03T05:45:52ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/982458982458VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose TissueRaphaela Schlich0Miriam Willems1Sabrina Greulich2Florian Ruppe3Wolfram Trudo Knoefel4D. Margriet Ouwens5Bujar Maxhera6Artur Lichtenberg7Jürgen Eckel8Henrike Sell9Paul-Langerhans-Group, Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyPaul-Langerhans-Group, Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanySignal Transduction, Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, 40225 Düsseldorf, GermanyDepartment of General-, Visceral-, and Pediatric Surgery, Heinrich-Heine-University and University Hospital Düsseldorf, 40225 Düsseldorf, GermanyDepartment of General-, Visceral-, and Pediatric Surgery, Heinrich-Heine-University and University Hospital Düsseldorf, 40225 Düsseldorf, GermanySignal Transduction, Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, 40225 Düsseldorf, GermanyDepartment of Cardiovascular Surgery, Düsseldorf University Hospital, 40225 Düsseldorf, GermanyDepartment of Cardiovascular Surgery, Düsseldorf University Hospital, 40225 Düsseldorf, GermanyPaul-Langerhans-Group, Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyPaul-Langerhans-Group, Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyAdipose tissue secrets adipokines and fatty acids, which may contribute to obesity-associated vascular dysfunction and cardiovascular risk. This study investigated which factors are responsible for the synergistic effect of adipokine and oleic acid- (OA-) induced proliferation of human vascular smooth muscle cells (VSMC). Adipocyte-conditioned medium (CM) from human adipocytes induces proliferation of VSMC in correlation to its vascular endothelial growth factor (VEGF) content. CM increases VEGF-receptor (VEGF-R) 1 and 2 expression and VEGF secretion of VSMC, while OA only stimulates VEGF secretion. VEGF neutralization abrogates CM- and OA-induced proliferation and considerably reduces proliferation induced by CM and OA in combination. VEGF release is higher from visceral adipose tissue (VAT) of obese subjects compared to subcutaneous adipose tissue (SAT) and VAT from lean controls. Furthermore, VEGF release from VAT correlates with its proliferative effect. Perivascular adipose tissue (PAT) from type 2 diabetic patients releases significantly higher amounts of VEGF and induces stronger proliferation of VSMC as compared to SAT and SAT/PAT of nondiabetics. In conclusion, VEGF is mediating CM-induced proliferation of VSMC. As this adipokine is released in high amounts from VAT of obese patients and PAT of diabetic patients, VEGF might link adipose tissue inflammation to increased VSMC proliferation.http://dx.doi.org/10.1155/2013/982458 |
| spellingShingle | Raphaela Schlich Miriam Willems Sabrina Greulich Florian Ruppe Wolfram Trudo Knoefel D. Margriet Ouwens Bujar Maxhera Artur Lichtenberg Jürgen Eckel Henrike Sell VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue Mediators of Inflammation |
| title | VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue |
| title_full | VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue |
| title_fullStr | VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue |
| title_full_unstemmed | VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue |
| title_short | VEGF in the Crosstalk between Human Adipocytes and Smooth Muscle Cells: Depot-Specific Release from Visceral and Perivascular Adipose Tissue |
| title_sort | vegf in the crosstalk between human adipocytes and smooth muscle cells depot specific release from visceral and perivascular adipose tissue |
| url | http://dx.doi.org/10.1155/2013/982458 |
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