Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in Dogs
Phylogenetic analyses indicate that canine influenza viruses (CIVs) (H3N8) evolved from contemporary equine influenza virus (EIV). Despite the genetic relatedness of EIV and CIV, recent evidence suggests that CIV is unable to infect, replicate, and spread among susceptible horses. To determine wheth...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2013/874521 |
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| author | Heidi L. Pecoraro Susi Bennett Kristina Garretson Ayshea M. Quintana Katharine F. Lunn Gabriele A. Landolt |
| author_facet | Heidi L. Pecoraro Susi Bennett Kristina Garretson Ayshea M. Quintana Katharine F. Lunn Gabriele A. Landolt |
| author_sort | Heidi L. Pecoraro |
| collection | DOAJ |
| description | Phylogenetic analyses indicate that canine influenza viruses (CIVs) (H3N8) evolved from contemporary equine influenza virus (EIV). Despite the genetic relatedness of EIV and CIV, recent evidence suggests that CIV is unable to infect, replicate, and spread among susceptible horses. To determine whether equine H3N8 viruses have equally lost the ability to infect, cause disease, and spread among dogs, we evaluated the infectivity and transmissibility of a recent Florida sublineage EIV isolate in dogs. Clinical signs, nasal virus shedding, and serological responses were monitored in dogs for 21 days after inoculation. Real-time reverse transcription-PCR and hemagglutination inhibition assays showed that both the viruses have maintained the ability to infect and replicate in dogs and result in seroconversion. Transmission of EIV from infected to sentinel dogs, however, was restricted. Furthermore, both CIV and EIV exhibited similar sialic acid-α2,3-gal receptor-binding preferences upon solid-phase binding assays. The results of the in vivo experiments reported here suggesting that dogs are susceptible to EIV and previous reports by members of our laboratory showing limited CIV infection in horses have been mirrored in CIV and EIV infections studies in primary canine and equine respiratory epithelial cells. |
| format | Article |
| id | doaj-art-ed3f1cde13bf4dbab40dab9729a27cbe |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-ed3f1cde13bf4dbab40dab9729a27cbe2025-02-03T01:02:09ZengWileyVeterinary Medicine International2090-81132042-00482013-01-01201310.1155/2013/874521874521Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in DogsHeidi L. Pecoraro0Susi Bennett1Kristina Garretson2Ayshea M. Quintana3Katharine F. Lunn4Gabriele A. Landolt5Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biological Sciences, Colorado State University, 300 West Drake Road, Campus Delivery 1678, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biological Sciences, Colorado State University, 300 West Drake Road, Campus Delivery 1678, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biological Sciences, Colorado State University, 300 West Drake Road, Campus Delivery 1678, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biological Sciences, Colorado State University, 300 West Drake Road, Campus Delivery 1678, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biological Sciences, Colorado State University, 300 West Drake Road, Campus Delivery 1678, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biological Sciences, Colorado State University, 300 West Drake Road, Campus Delivery 1678, Fort Collins, CO 80523, USAPhylogenetic analyses indicate that canine influenza viruses (CIVs) (H3N8) evolved from contemporary equine influenza virus (EIV). Despite the genetic relatedness of EIV and CIV, recent evidence suggests that CIV is unable to infect, replicate, and spread among susceptible horses. To determine whether equine H3N8 viruses have equally lost the ability to infect, cause disease, and spread among dogs, we evaluated the infectivity and transmissibility of a recent Florida sublineage EIV isolate in dogs. Clinical signs, nasal virus shedding, and serological responses were monitored in dogs for 21 days after inoculation. Real-time reverse transcription-PCR and hemagglutination inhibition assays showed that both the viruses have maintained the ability to infect and replicate in dogs and result in seroconversion. Transmission of EIV from infected to sentinel dogs, however, was restricted. Furthermore, both CIV and EIV exhibited similar sialic acid-α2,3-gal receptor-binding preferences upon solid-phase binding assays. The results of the in vivo experiments reported here suggesting that dogs are susceptible to EIV and previous reports by members of our laboratory showing limited CIV infection in horses have been mirrored in CIV and EIV infections studies in primary canine and equine respiratory epithelial cells.http://dx.doi.org/10.1155/2013/874521 |
| spellingShingle | Heidi L. Pecoraro Susi Bennett Kristina Garretson Ayshea M. Quintana Katharine F. Lunn Gabriele A. Landolt Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in Dogs Veterinary Medicine International |
| title | Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in Dogs |
| title_full | Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in Dogs |
| title_fullStr | Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in Dogs |
| title_full_unstemmed | Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in Dogs |
| title_short | Comparison of the Infectivity and Transmission of Contemporary Canine and Equine H3N8 Influenza Viruses in Dogs |
| title_sort | comparison of the infectivity and transmission of contemporary canine and equine h3n8 influenza viruses in dogs |
| url | http://dx.doi.org/10.1155/2013/874521 |
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