De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative Analyses
Known to be rich in β-glucan, Sparassis latifolia (S. latifolia) is a valuable edible fungus cultivated in East Asia. A few studies have suggested that S. latifolia is effective on antidiabetic, antihypertension, antitumor, and antiallergen medications. However, it is still unclear genetically why t...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/1857170 |
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| author | Donglai Xiao Lu Ma Chi Yang Zhenghe Ying Xiaoling Jiang Yan-quan Lin |
| author_facet | Donglai Xiao Lu Ma Chi Yang Zhenghe Ying Xiaoling Jiang Yan-quan Lin |
| author_sort | Donglai Xiao |
| collection | DOAJ |
| description | Known to be rich in β-glucan, Sparassis latifolia (S. latifolia) is a valuable edible fungus cultivated in East Asia. A few studies have suggested that S. latifolia is effective on antidiabetic, antihypertension, antitumor, and antiallergen medications. However, it is still unclear genetically why the fungus has these medical effects, which has become a key bottleneck for its further applications. To provide a better understanding of this fungus, we sequenced its whole genome, which has a total size of 48.13 megabases (Mb) and contains 12,471 predicted gene models. We then performed comparative and phylogenetic analyses, which indicate that S. latifolia is closely related to a few species in the antrodia clade including Fomitopsis pinicola, Wolfiporia cocos, Postia placenta, and Antrodia sinuosa. Finally, we annotated the predicted genes. Interestingly, the S. latifolia genome encodes most enzymes involved in carbohydrate and glycoconjugate metabolism and is also enriched in genes encoding enzymes critical to secondary metabolite biosynthesis and involved in indole, terpene, and type I polyketide pathways. As a conclusion, the genome content of S. latifolia sheds light on its genetic basis of the reported medicinal properties and could also be used as a reference genome for comparative studies on fungi. |
| format | Article |
| id | doaj-art-ecbd666a5f9b4bff87c0a1e8907c721d |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-ecbd666a5f9b4bff87c0a1e8907c721d2025-02-03T01:26:36ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932018-01-01201810.1155/2018/18571701857170De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative AnalysesDonglai Xiao0Lu Ma1Chi Yang2Zhenghe Ying3Xiaoling Jiang4Yan-quan Lin5The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, ChinaThe Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, ChinaThe Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, ChinaThe Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, ChinaThe Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, ChinaThe Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, ChinaKnown to be rich in β-glucan, Sparassis latifolia (S. latifolia) is a valuable edible fungus cultivated in East Asia. A few studies have suggested that S. latifolia is effective on antidiabetic, antihypertension, antitumor, and antiallergen medications. However, it is still unclear genetically why the fungus has these medical effects, which has become a key bottleneck for its further applications. To provide a better understanding of this fungus, we sequenced its whole genome, which has a total size of 48.13 megabases (Mb) and contains 12,471 predicted gene models. We then performed comparative and phylogenetic analyses, which indicate that S. latifolia is closely related to a few species in the antrodia clade including Fomitopsis pinicola, Wolfiporia cocos, Postia placenta, and Antrodia sinuosa. Finally, we annotated the predicted genes. Interestingly, the S. latifolia genome encodes most enzymes involved in carbohydrate and glycoconjugate metabolism and is also enriched in genes encoding enzymes critical to secondary metabolite biosynthesis and involved in indole, terpene, and type I polyketide pathways. As a conclusion, the genome content of S. latifolia sheds light on its genetic basis of the reported medicinal properties and could also be used as a reference genome for comparative studies on fungi.http://dx.doi.org/10.1155/2018/1857170 |
| spellingShingle | Donglai Xiao Lu Ma Chi Yang Zhenghe Ying Xiaoling Jiang Yan-quan Lin De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative Analyses Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative Analyses |
| title_full | De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative Analyses |
| title_fullStr | De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative Analyses |
| title_full_unstemmed | De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative Analyses |
| title_short | De Novo Sequencing of a Sparassis latifolia Genome and Its Associated Comparative Analyses |
| title_sort | de novo sequencing of a sparassis latifolia genome and its associated comparative analyses |
| url | http://dx.doi.org/10.1155/2018/1857170 |
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