Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer
Tumor necrosis factor (TNF-α) and inflammatory cytokine (IL-6) play a vital role in various cellular incidents such as the proliferation and death of cells during carcinogenesis. Hence, regulation of these biomarkers could be a promising tool for controlling tumor progression using nanoformulations....
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2022/6181448 |
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| author | Ahmed A. H. Abdellatif Ahmed Abdelfattah Abdellatif Bouazzaoui Shaaban K. Osman Issa Saad Al-Moraya Abdulaziz M. Saleh Showail Mansour Alsharidah Ashraf Aboelela Osamah Al Rugaie Tarek M. Faris Hesham M. Tawfeek |
| author_facet | Ahmed A. H. Abdellatif Ahmed Abdelfattah Abdellatif Bouazzaoui Shaaban K. Osman Issa Saad Al-Moraya Abdulaziz M. Saleh Showail Mansour Alsharidah Ashraf Aboelela Osamah Al Rugaie Tarek M. Faris Hesham M. Tawfeek |
| author_sort | Ahmed A. H. Abdellatif |
| collection | DOAJ |
| description | Tumor necrosis factor (TNF-α) and inflammatory cytokine (IL-6) play a vital role in various cellular incidents such as the proliferation and death of cells during carcinogenesis. Hence, regulation of these biomarkers could be a promising tool for controlling tumor progression using nanoformulations. Silver nanoparticles-poly (vinyl pyrrolidone) (AgNPs-PVP) were prepared using the reduction of silver nitrate and stabilized with PVP. They are characterized through yield percentage, UV-VIS, FT-IR, size, charge, and morphology. The obtained AgNPs were tested for anticancer activity against prostate cancer (PC 3) and human skin fibroblast (HFS) cell lines. Moreover, biomarker-based confirmations like TNF-α and IL-6 were estimated. The synthesized AgNPs-PVP were stable, spherical in shape, with particle sizes of 122.33 ± 17.61 nm, a polydispersity index of 0.49 ± 0.07, and a negative surface charge of −19.23 ± 0.61 mV. In vitro cytotoxicity testing showed the AgNPs-PVP exhibited antiproliferation properties in PC3 in a dose-dependent manner. In addition, when compared to control cells, AgNPs-PVP has lower TNF-α with a significant value (∗p<0.05); the value reached 16.84 ± 0.71 pg/ml versus 20.81 ± 0.44 pg/ml, respectively. In addition, HSF cells showed a high level of reduction (∗∗∗p<0.001) in IL-6 production. This study suggested that AgNPs-PVP could be a possible therapeutic agent for human prostate cancer and anti-IL-6 in cancerous and noncancerous cells. Further studies will be performed to investigate the effect of AgNPs-PVP in different types of cancer. |
| format | Article |
| id | doaj-art-ec6e878e1b6b4472ad842e8622514c38 |
| institution | Kabale University |
| issn | 1687-479X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-ec6e878e1b6b4472ad842e8622514c382025-02-03T01:22:49ZengWileyBioinorganic Chemistry and Applications1687-479X2022-01-01202210.1155/2022/6181448Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate CancerAhmed A. H. Abdellatif0Ahmed Abdelfattah1Abdellatif Bouazzaoui2Shaaban K. Osman3Issa Saad Al-Moraya4Abdulaziz M. Saleh Showail5Mansour Alsharidah6Ashraf Aboelela7Osamah Al Rugaie8Tarek M. Faris9Hesham M. Tawfeek10Department of PharmaceuticsDepartment of Industrial PharmacyDepartment of Medical GeneticsDepartment of Pharmaceutics and Pharmaceutical TechnologyClinical ToxicologyDepartment of UrologyDepartment of PhysiologyDepartment of Pharmaceutical ChemistryDepartment of Basic Medical SciencesDepartment of Pharmaceutics and Industrial PharmacyDepartment of Industrial PharmacyTumor necrosis factor (TNF-α) and inflammatory cytokine (IL-6) play a vital role in various cellular incidents such as the proliferation and death of cells during carcinogenesis. Hence, regulation of these biomarkers could be a promising tool for controlling tumor progression using nanoformulations. Silver nanoparticles-poly (vinyl pyrrolidone) (AgNPs-PVP) were prepared using the reduction of silver nitrate and stabilized with PVP. They are characterized through yield percentage, UV-VIS, FT-IR, size, charge, and morphology. The obtained AgNPs were tested for anticancer activity against prostate cancer (PC 3) and human skin fibroblast (HFS) cell lines. Moreover, biomarker-based confirmations like TNF-α and IL-6 were estimated. The synthesized AgNPs-PVP were stable, spherical in shape, with particle sizes of 122.33 ± 17.61 nm, a polydispersity index of 0.49 ± 0.07, and a negative surface charge of −19.23 ± 0.61 mV. In vitro cytotoxicity testing showed the AgNPs-PVP exhibited antiproliferation properties in PC3 in a dose-dependent manner. In addition, when compared to control cells, AgNPs-PVP has lower TNF-α with a significant value (∗p<0.05); the value reached 16.84 ± 0.71 pg/ml versus 20.81 ± 0.44 pg/ml, respectively. In addition, HSF cells showed a high level of reduction (∗∗∗p<0.001) in IL-6 production. This study suggested that AgNPs-PVP could be a possible therapeutic agent for human prostate cancer and anti-IL-6 in cancerous and noncancerous cells. Further studies will be performed to investigate the effect of AgNPs-PVP in different types of cancer.http://dx.doi.org/10.1155/2022/6181448 |
| spellingShingle | Ahmed A. H. Abdellatif Ahmed Abdelfattah Abdellatif Bouazzaoui Shaaban K. Osman Issa Saad Al-Moraya Abdulaziz M. Saleh Showail Mansour Alsharidah Ashraf Aboelela Osamah Al Rugaie Tarek M. Faris Hesham M. Tawfeek Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer Bioinorganic Chemistry and Applications |
| title | Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer |
| title_full | Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer |
| title_fullStr | Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer |
| title_full_unstemmed | Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer |
| title_short | Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer |
| title_sort | silver nanoparticles stabilized by poly vinyl pyrrolidone with potential anticancer activity towards prostate cancer |
| url | http://dx.doi.org/10.1155/2022/6181448 |
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