Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular Chondrocytes
Objectives. The objective of this paper is to determine if hyaluronan affects bupivacaine’s anesthetic function. Methods. Whole cell patch clamp recordings were performed on bovine articular chondrocytes cultured in 60 mm dishes. The chondrocytes were treated with phosphate-buffered saline (control...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Advances in Orthopedics |
| Online Access: | http://dx.doi.org/10.1155/2012/361534 |
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| author | William Hester Jinnan Yang Guo-Yong Wang Sen Liu Michael J. O'Brien Felix H. Savoie Zongbing You |
| author_facet | William Hester Jinnan Yang Guo-Yong Wang Sen Liu Michael J. O'Brien Felix H. Savoie Zongbing You |
| author_sort | William Hester |
| collection | DOAJ |
| description | Objectives. The objective of this paper is to determine if hyaluronan affects bupivacaine’s anesthetic function. Methods. Whole cell patch clamp recordings were performed on bovine articular chondrocytes cultured in 60 mm dishes. The chondrocytes were treated with phosphate-buffered saline (control group), 7.5 mg/mL hyaluronan (Orthovisc), 0.25% bupivacaine, or a mixture of 7.5 mg/mL hyaluronan and 0.25% bupivacaine. Outward currents were elicited by step depolarization from −90 mV to 150 mV with 5 mV increments and holding for 200 ms. Results. The amplitude of outward currents elicited at 150 mV was 607.1±135.4 pA (mean ± standard error) in the chondrocytes treated with phosphate buffered saline, 550.0±194.9 pA in the chondrocytes treated with hyaluronan, 18.4±8.3 pA in the chondrocytes treated with bupivacaine, and 12.8±2.6 pA in the chondrocytes treated with a mixture of hyaluronan and bupivacaine. Conclusion. Hyaluronan does not affect bupivacaine’s inhibitory action on the potassium channel activities in bovine articular chondrocytes. This finding suggests that intra-articular injection of a mixture of hyaluronan and bupivacaine may not affect the anesthetic effects of bupivacaine. |
| format | Article |
| id | doaj-art-eb20d38a80254c1d8569e3152dfbf38c |
| institution | Kabale University |
| issn | 2090-3464 2090-3472 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Orthopedics |
| spelling | doaj-art-eb20d38a80254c1d8569e3152dfbf38c2025-02-03T05:51:52ZengWileyAdvances in Orthopedics2090-34642090-34722012-01-01201210.1155/2012/361534361534Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular ChondrocytesWilliam Hester0Jinnan Yang1Guo-Yong Wang2Sen Liu3Michael J. O'Brien4Felix H. Savoie5Zongbing You6Department of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Orthopaedic Surgery and Tulane Institute of Sports Medicine, Tulane University School of Medicine, New Orleans, LA, USADepartment of Orthopaedic Surgery and Tulane Institute of Sports Medicine, Tulane University School of Medicine, New Orleans, LA, USADepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA 70112, USAObjectives. The objective of this paper is to determine if hyaluronan affects bupivacaine’s anesthetic function. Methods. Whole cell patch clamp recordings were performed on bovine articular chondrocytes cultured in 60 mm dishes. The chondrocytes were treated with phosphate-buffered saline (control group), 7.5 mg/mL hyaluronan (Orthovisc), 0.25% bupivacaine, or a mixture of 7.5 mg/mL hyaluronan and 0.25% bupivacaine. Outward currents were elicited by step depolarization from −90 mV to 150 mV with 5 mV increments and holding for 200 ms. Results. The amplitude of outward currents elicited at 150 mV was 607.1±135.4 pA (mean ± standard error) in the chondrocytes treated with phosphate buffered saline, 550.0±194.9 pA in the chondrocytes treated with hyaluronan, 18.4±8.3 pA in the chondrocytes treated with bupivacaine, and 12.8±2.6 pA in the chondrocytes treated with a mixture of hyaluronan and bupivacaine. Conclusion. Hyaluronan does not affect bupivacaine’s inhibitory action on the potassium channel activities in bovine articular chondrocytes. This finding suggests that intra-articular injection of a mixture of hyaluronan and bupivacaine may not affect the anesthetic effects of bupivacaine.http://dx.doi.org/10.1155/2012/361534 |
| spellingShingle | William Hester Jinnan Yang Guo-Yong Wang Sen Liu Michael J. O'Brien Felix H. Savoie Zongbing You Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular Chondrocytes Advances in Orthopedics |
| title | Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular Chondrocytes |
| title_full | Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular Chondrocytes |
| title_fullStr | Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular Chondrocytes |
| title_full_unstemmed | Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular Chondrocytes |
| title_short | Hyaluronan Does Not Affect Bupivacaine’s Inhibitory Action on Voltage-Gated Potassium Channel Activities in Bovine Articular Chondrocytes |
| title_sort | hyaluronan does not affect bupivacaine s inhibitory action on voltage gated potassium channel activities in bovine articular chondrocytes |
| url | http://dx.doi.org/10.1155/2012/361534 |
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