Truncating the C terminus of formate dehydrogenase leads to improved preference to nicotinamide cytosine dinucleotide

Truncating the C terminus of formate dehydrogenase leads to improved preference to nicotinamide cytosine dinucleotide

Abstract Formate dehydrogenase (FDH) is widely applied in regeneration of redox cofactors. There are continuing interests to engineer FDH for improved catalytic activity and cofactor preference. In the crystal structure of FDH from Pseudomonas sp. 101 (pseFDH), the C terminus with 9 amino acid resid...

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Bibliographic Details
Main Authors: Xiaojia Guo, Xueying Wang, Yinghan Hu, Lingyun Zhang, Zongbao K. Zhao
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
Subjects:
Formate dehydrogenase
Directed evolution
Cofactor preference
C terminus
Non-natural cofactor
Online Access:https://doi.org/10.1038/s41598-024-79885-z
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https://doi.org/10.1038/s41598-024-79885-z

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