Validation of quantitative determination methods of diphenhyramine tablets by high performance liquid chromatography
Aim. In order to quantify diphenhydramine tablets the methods based on reverse phase high-performance liquid chromatography with UV-spectrometry detection at 254 nm (HPLC-UV) have been used. Methods and results. The mobile phase used a mixture of a phosphate buffer: acetonitrile (80:20). The prop...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zaporizhzhia State Medical and Pharmaceutical University
2015-04-01
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| Series: | Zaporožskij Medicinskij Žurnal |
| Subjects: | |
| Online Access: | http://zmj.zsmu.edu.ua/article/view/42122/38450 |
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| Summary: | Aim. In order to quantify diphenhydramine tablets the methods based on reverse phase high-performance liquid chromatography with UV-spectrometry detection at 254 nm (HPLC-UV) have been used.
Methods and results. The mobile phase used a mixture of a phosphate buffer: acetonitrile (80:20). The proposed method is applied to the two dosage forms of diphenhydramine – tablets to 0.1 and 0.05, produced by various Ukrainian pharmaceutical companies. The main characteristics are validated by Pharmacopoeia of Ukraine.
Conclusion. According to the experimental data, the technique can be correctly reproduced, and is suitable for use in pharmaceutical and forensic analytic laboratories. |
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| ISSN: | 2306-4145 2310-1210 |