In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancer

Abstract A significant percentage of colorectal cancer (CRC) diagnoses are associated with overexpression of the MAPK14 gene MAPK14 gene or p38 MAPK. It is overexpressed in most cases of colorectal cancer (CRC) and is an important factor for tumor development. CRC is among the most common causes of...

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Main Authors: Meerab Khalid, Rana Muhammad Mateen, Mohsin Javed, Muhammad Ali, Mohammed Arif Nadeem Saqab, Rukhsana Parveen, Ahmed Asimov, Safura Bibi, Ali Bahadur, Shahid Iqbal, Sajid Mahmood, Salah Knani, Abeer Ahmed Alghamdi, Randa A. Althobiti, Lamiaa Galal Amin
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-05807-2
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author Meerab Khalid
Rana Muhammad Mateen
Mohsin Javed
Muhammad Ali
Mohammed Arif Nadeem Saqab
Rukhsana Parveen
Ahmed Asimov
Safura Bibi
Ali Bahadur
Shahid Iqbal
Sajid Mahmood
Salah Knani
Abeer Ahmed Alghamdi
Randa A. Althobiti
Lamiaa Galal Amin
author_facet Meerab Khalid
Rana Muhammad Mateen
Mohsin Javed
Muhammad Ali
Mohammed Arif Nadeem Saqab
Rukhsana Parveen
Ahmed Asimov
Safura Bibi
Ali Bahadur
Shahid Iqbal
Sajid Mahmood
Salah Knani
Abeer Ahmed Alghamdi
Randa A. Althobiti
Lamiaa Galal Amin
author_sort Meerab Khalid
collection DOAJ
description Abstract A significant percentage of colorectal cancer (CRC) diagnoses are associated with overexpression of the MAPK14 gene MAPK14 gene or p38 MAPK. It is overexpressed in most cases of colorectal cancer (CRC) and is an important factor for tumor development. CRC is among the most common causes of cancer-related deaths globally It is potent to target this gene for therapeutic intervention because it is important for tumor immune evasion, cell proliferation, survival, and resistance to therapy. By developing treatment techniques that target MAPK14 is a promising approach. In this study, we employed in silico methods to evaluate the potential of phytochemicals as inhibitors of MAPK14, which is overexpressed in CRC. We also included Fruquintinib, an FDA-approved anticancer drug, as a reference compound for molecular docking with MAPK14. Compounds meeting acceptable ADMET profiles were further analyzed using molecular docking, DFT, and MD simulations to identify potential MAPK14 inhibitors. Compound 1 (CID: 44586092) was discovered in our investigation to be a potential therapy for CRC associated with MAPK14.
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spelling doaj-art-eabb302945cc4d028a2c3c02ab10b8d72025-08-20T03:03:33ZengNature PortfolioScientific Reports2045-23222025-07-0115111710.1038/s41598-025-05807-2In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancerMeerab Khalid0Rana Muhammad Mateen1Mohsin Javed2Muhammad Ali3Mohammed Arif Nadeem Saqab4Rukhsana Parveen5Ahmed Asimov6Safura Bibi7Ali Bahadur8Shahid Iqbal9Sajid Mahmood10Salah Knani11Abeer Ahmed Alghamdi12Randa A. Althobiti13Lamiaa Galal Amin14Department of Life Sciences, School of Science, University of Management and Technology (UMT)Department of Life Sciences, School of Science, University of Management and Technology (UMT)Department of Chemistry, School of Science, University of Management and TechnologyDepartment of Life Sciences, School of Science, University of Management and Technology (UMT)Department of Chemistry, School of Science, University of Management and TechnologyCentre for Applied Molecular Biology, University of the PunjabComposite Materials Scientific Research Center of Azerbaijan, State University of EconomicsDepartment of Botany, University of AgricultureNanomaterials Research Center, Department of Chemistry, College of Science, Mathematics, and Technology, Wenzhou-Kean UniversityNottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo ChinaLow Dimensional Materials Research Center, Khazar UniversityCenter for Scientific Research and Entrepreneurship, Northern Border UniversityDepartment of Physics, College of Science, Princess Nourah bint Abdulrahman UniversityDepartment of Chemistry, College of Science, University of BishaDepartment of Physics, College of Science, Northern Border UniversityAbstract A significant percentage of colorectal cancer (CRC) diagnoses are associated with overexpression of the MAPK14 gene MAPK14 gene or p38 MAPK. It is overexpressed in most cases of colorectal cancer (CRC) and is an important factor for tumor development. CRC is among the most common causes of cancer-related deaths globally It is potent to target this gene for therapeutic intervention because it is important for tumor immune evasion, cell proliferation, survival, and resistance to therapy. By developing treatment techniques that target MAPK14 is a promising approach. In this study, we employed in silico methods to evaluate the potential of phytochemicals as inhibitors of MAPK14, which is overexpressed in CRC. We also included Fruquintinib, an FDA-approved anticancer drug, as a reference compound for molecular docking with MAPK14. Compounds meeting acceptable ADMET profiles were further analyzed using molecular docking, DFT, and MD simulations to identify potential MAPK14 inhibitors. Compound 1 (CID: 44586092) was discovered in our investigation to be a potential therapy for CRC associated with MAPK14.https://doi.org/10.1038/s41598-025-05807-2In silicoMAPK14CRC (CRC)FruquintinibDFTMD
spellingShingle Meerab Khalid
Rana Muhammad Mateen
Mohsin Javed
Muhammad Ali
Mohammed Arif Nadeem Saqab
Rukhsana Parveen
Ahmed Asimov
Safura Bibi
Ali Bahadur
Shahid Iqbal
Sajid Mahmood
Salah Knani
Abeer Ahmed Alghamdi
Randa A. Althobiti
Lamiaa Galal Amin
In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancer
Scientific Reports
In silico
MAPK14
CRC (CRC)
Fruquintinib
DFT
MD
title In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancer
title_full In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancer
title_fullStr In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancer
title_full_unstemmed In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancer
title_short In-silico analysis of potential phytochemicals targeting mitogen activating protein kinase-14 (MAPK14) gene in colorectal cancer
title_sort in silico analysis of potential phytochemicals targeting mitogen activating protein kinase 14 mapk14 gene in colorectal cancer
topic In silico
MAPK14
CRC (CRC)
Fruquintinib
DFT
MD
url https://doi.org/10.1038/s41598-025-05807-2
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