Scalable production and purification of engineered ARRDC1-mediated microvesicles in a HEK293 suspension cell system

Scalable production and purification of engineered ARRDC1-mediated microvesicles in a HEK293 suspension cell system

Abstract Engineering of human ARRDC1-mediated microvesicles (ARMMs) as non-viral vehicles for delivery of gene therapies bears the potential to enable novel therapeutic paradigms. We evaluated two scalable strategies to generate ARMMs loaded with protein cargo, by transient transfection or stable ce...

Full description

Saved in:
Bibliographic Details
Main Authors: Kristin Luther, Ali Navaei, Leah Gens, Carson Semple, Pearl Moharil, Ilaria Passalacqua, Komal Vyas, Qiyu Wang, Shu-Lin Liu, Lucy Sun, Senthil Ramaswamy, Davide Zocco, Joseph F. Nabhan
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
Subjects:
Extracellular vesicles
ARRDC1-mediated microvesicles
ARMMs
5B8
Transient transfection
Stable producer cell line
Online Access:https://doi.org/10.1038/s41598-025-87674-5
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://doi.org/10.1038/s41598-025-87674-5

Similar Items