Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress
Atrogin-1, a muscle-specific ubiquitin ligase, is integral to the regulation of protein degradation and plays a pivotal role in the targeted degradation of critical muscle signaling proteins, which can lead to skeletal muscle dysfunction. High salt stress is known to induce muscle atrophy and alter...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006306 |
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| author | Yong-Juan Mo Yi-Li Gao Shi-Jjian Xu Bin Wang Jingping Wu Peng Yu Ming Li Xiangtao Kang Zhi-Qiang Han |
| author_facet | Yong-Juan Mo Yi-Li Gao Shi-Jjian Xu Bin Wang Jingping Wu Peng Yu Ming Li Xiangtao Kang Zhi-Qiang Han |
| author_sort | Yong-Juan Mo |
| collection | DOAJ |
| description | Atrogin-1, a muscle-specific ubiquitin ligase, is integral to the regulation of protein degradation and plays a pivotal role in the targeted degradation of critical muscle signaling proteins, which can lead to skeletal muscle dysfunction. High salt stress is known to induce muscle atrophy and alter the physical and chemical properties of muscle in cultured fish. Conversely, an optimal salinity level can enhance muscle quality. The Russian sturgeon (Acipenser gueldenstaedtii), an anadromous species of significant economic value, is potentially adaptable to brackish water environments, yet the underlying regulatory mechanisms remain elusive. To elucidate the regulatory mechanism of Atrogin-1 in response to high salt stress in Russian sturgeon, we conducted cDNA cloning and expression analysis. The cDNA encoding the Russian sturgeon protein Atrogin-1 was successfully cloned, comprising 1472 bp and encoding 353 amino acid residues (with an open reading frame of 1062 bp). Utilizing the sequence of Russian sturgeon atrogin-1, we designed novel primers and a specific anti-peptide antibody for our study, confirming that atrogin-1 is selectively expressed in skeletal muscle and heart tissues. The sturgeons were then allocated into four experimental groups for salt stress exposure; one control group was maintained in freshwater, while the others were subjected to salinities of 8 ‰, 16 ‰, and 24 ‰, respectively. Quantitative PCR (qPCR) and western blot analysis were employed to assess Atrogin-1 expression levels under high salt stress. A significant upregulation of atrogin-1 mRNA was observed at salinities of 16 ‰ and 24 ‰ compared to the control group. Similarly, protein expression levels of Atrogin-1 were markedly elevated under the same salinity conditions. Our findings indicate that the modulation of Atrogin-1 expression is a sensitive indicator of the response to high salt stress. This research is anticipated to inform aquaculture practices and shed light on the selection of germplasm resources for the development of salt-tolerant Russian sturgeon strains. |
| format | Article |
| id | doaj-art-e9fb256a907b400da432f96d24554589 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-e9fb256a907b400da432f96d245545892025-02-06T05:12:04ZengElsevierAquaculture Reports2352-51342025-03-0140102542Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stressYong-Juan Mo0Yi-Li Gao1Shi-Jjian Xu2Bin Wang3Jingping Wu4Peng Yu5Ming Li6Xiangtao Kang7Zhi-Qiang Han8Fishery College, Zhejiang Ocean University, Zhoushan 316022, ChinaNingbo Key Laboratory of Agricultural Germplasm Resources Mining and Environmental Regulation, College of Science and Technology, Ningbo University, Cixi 315300, China; Quzhou Sturgeon Aquatic Food Science and Technology Development Co., Ltd., Quzhou 324002, China; Corresponding author at: College of Science and Technology, Ningbo University, No. 521, Wenwei Road, Cixi 315300, China.Quzhou Sturgeon Aquatic Food Science and Technology Development Co., Ltd., Quzhou 324002, China; Key Laboratory of Sturgeon Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hangzhou 311799, China; Corresponding author at: Quzhou Sturgeon Aquatic Food Science and Technology Development Co., Ltd., No. 1, Xunlong Road, Quzhou 324002, China.Quzhou Sturgeon Aquatic Food Science and Technology Development Co., Ltd., Quzhou 324002, China; Key Laboratory of Sturgeon Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hangzhou 311799, ChinaQuzhou Sturgeon Aquatic Food Science and Technology Development Co., Ltd., Quzhou 324002, China; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaFishery College, Zhejiang Ocean University, Zhoushan 316022, ChinaAtrogin-1, a muscle-specific ubiquitin ligase, is integral to the regulation of protein degradation and plays a pivotal role in the targeted degradation of critical muscle signaling proteins, which can lead to skeletal muscle dysfunction. High salt stress is known to induce muscle atrophy and alter the physical and chemical properties of muscle in cultured fish. Conversely, an optimal salinity level can enhance muscle quality. The Russian sturgeon (Acipenser gueldenstaedtii), an anadromous species of significant economic value, is potentially adaptable to brackish water environments, yet the underlying regulatory mechanisms remain elusive. To elucidate the regulatory mechanism of Atrogin-1 in response to high salt stress in Russian sturgeon, we conducted cDNA cloning and expression analysis. The cDNA encoding the Russian sturgeon protein Atrogin-1 was successfully cloned, comprising 1472 bp and encoding 353 amino acid residues (with an open reading frame of 1062 bp). Utilizing the sequence of Russian sturgeon atrogin-1, we designed novel primers and a specific anti-peptide antibody for our study, confirming that atrogin-1 is selectively expressed in skeletal muscle and heart tissues. The sturgeons were then allocated into four experimental groups for salt stress exposure; one control group was maintained in freshwater, while the others were subjected to salinities of 8 ‰, 16 ‰, and 24 ‰, respectively. Quantitative PCR (qPCR) and western blot analysis were employed to assess Atrogin-1 expression levels under high salt stress. A significant upregulation of atrogin-1 mRNA was observed at salinities of 16 ‰ and 24 ‰ compared to the control group. Similarly, protein expression levels of Atrogin-1 were markedly elevated under the same salinity conditions. Our findings indicate that the modulation of Atrogin-1 expression is a sensitive indicator of the response to high salt stress. This research is anticipated to inform aquaculture practices and shed light on the selection of germplasm resources for the development of salt-tolerant Russian sturgeon strains.http://www.sciencedirect.com/science/article/pii/S2352513424006306Atrogin-1High salt stressAcipenser gueldenstaedtiiSelective breeding |
| spellingShingle | Yong-Juan Mo Yi-Li Gao Shi-Jjian Xu Bin Wang Jingping Wu Peng Yu Ming Li Xiangtao Kang Zhi-Qiang Han Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress Aquaculture Reports Atrogin-1 High salt stress Acipenser gueldenstaedtii Selective breeding |
| title | Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress |
| title_full | Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress |
| title_fullStr | Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress |
| title_full_unstemmed | Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress |
| title_short | Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress |
| title_sort | molecular cloning and expression dynamics of atrogin 1 in muscle tissue of russian sturgeon acipenser gueldenstaedtii under high salt stress |
| topic | Atrogin-1 High salt stress Acipenser gueldenstaedtii Selective breeding |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006306 |
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