Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study
<b>Background</b>: Yeokwisan (YWS) is a standardised herbal formula for relieving functional dyspepsia symptoms. <b>Methods</b>: We explored the therapeutic value of YWS and its potential effects on gastritis. Its inhibitory effect on gastric mucosal damage and anti-inflammat...
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2025-01-01
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| author | Yun Mi Lee Kyuhyung Jo So Yeon Kim Chang-Seob Seo Eunjung Son Aejin Kim Dong-Seon Kim |
| author_facet | Yun Mi Lee Kyuhyung Jo So Yeon Kim Chang-Seob Seo Eunjung Son Aejin Kim Dong-Seon Kim |
| author_sort | Yun Mi Lee |
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| description | <b>Background</b>: Yeokwisan (YWS) is a standardised herbal formula for relieving functional dyspepsia symptoms. <b>Methods</b>: We explored the therapeutic value of YWS and its potential effects on gastritis. Its inhibitory effect on gastric mucosal damage and anti-inflammatory activity in animal models of alcohol- and restraint stress-induced gastritis were also examined. Gastric tissues of ICR mice treated with YWS (150 and 300 mg/kg) or famotidine (5 mg/kg) for 10 days were collected, and gastric lesions were quantified. The stomachs of C57BL/6 mice treated with YWS (150 and 300 mg/kg) or famotidine (5 mg/kg) for 23 days were collected, and gastric lesions were quantified. Blood samples were analysed for inflammation related factors and gastroprotective effects. <b>Results</b>: YWS (300 mg/kg) inhibited gastric damage by 42.33% in the EtOH-induced gastritis model and 75% in the restraint stress-induced gastritis model (compared to the control group). Pretreatment with YWS led to decreased levels of inflammatory factors (IL-1β, IL-6, and COX-2). YWS showed gastroprotective effects through histamine downregulation, while prostaglandin E2 (PGE2) and mucin were upregulated. The mRNA levels of H2R, M3R, CCK2R, and H<sup>+</sup>/K<sup>+</sup> ATPase were significantly decreased following treatment with YWS. <b>Conclusions:</b> YWS provides gastric protection through its anti-inflammatory properties, reduced histamine secretion, and enhanced release of mucosal defensive factors. |
| format | Article |
| id | doaj-art-e97e354c9c3d4c2c94038650fb3c2dba |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-e97e354c9c3d4c2c94038650fb3c2dba2025-01-24T13:45:08ZengMDPI AGPharmaceuticals1424-82472025-01-011814410.3390/ph18010044Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical StudyYun Mi Lee0Kyuhyung Jo1So Yeon Kim2Chang-Seob Seo3Eunjung Son4Aejin Kim5Dong-Seon Kim6KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of KoreaKM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of KoreaKM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea<b>Background</b>: Yeokwisan (YWS) is a standardised herbal formula for relieving functional dyspepsia symptoms. <b>Methods</b>: We explored the therapeutic value of YWS and its potential effects on gastritis. Its inhibitory effect on gastric mucosal damage and anti-inflammatory activity in animal models of alcohol- and restraint stress-induced gastritis were also examined. Gastric tissues of ICR mice treated with YWS (150 and 300 mg/kg) or famotidine (5 mg/kg) for 10 days were collected, and gastric lesions were quantified. The stomachs of C57BL/6 mice treated with YWS (150 and 300 mg/kg) or famotidine (5 mg/kg) for 23 days were collected, and gastric lesions were quantified. Blood samples were analysed for inflammation related factors and gastroprotective effects. <b>Results</b>: YWS (300 mg/kg) inhibited gastric damage by 42.33% in the EtOH-induced gastritis model and 75% in the restraint stress-induced gastritis model (compared to the control group). Pretreatment with YWS led to decreased levels of inflammatory factors (IL-1β, IL-6, and COX-2). YWS showed gastroprotective effects through histamine downregulation, while prostaglandin E2 (PGE2) and mucin were upregulated. The mRNA levels of H2R, M3R, CCK2R, and H<sup>+</sup>/K<sup>+</sup> ATPase were significantly decreased following treatment with YWS. <b>Conclusions:</b> YWS provides gastric protection through its anti-inflammatory properties, reduced histamine secretion, and enhanced release of mucosal defensive factors.https://www.mdpi.com/1424-8247/18/1/44Yeokwisangastritisalcoholrestraint stress |
| spellingShingle | Yun Mi Lee Kyuhyung Jo So Yeon Kim Chang-Seob Seo Eunjung Son Aejin Kim Dong-Seon Kim Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study Pharmaceuticals Yeokwisan gastritis alcohol restraint stress |
| title | Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study |
| title_full | Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study |
| title_fullStr | Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study |
| title_full_unstemmed | Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study |
| title_short | Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study |
| title_sort | yeokwisan standardised herbal formula enhancing gastric mucosal protection against gastric ulcers in mice a preclinical study |
| topic | Yeokwisan gastritis alcohol restraint stress |
| url | https://www.mdpi.com/1424-8247/18/1/44 |
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